D-阿洛糖对小鼠脑缺血再灌注血脑屏障影响  

Effect of D-allose on the blood-brain barrier following focal cerebral ischemia/reperfusion in mice

作　　者：黄涛[1] 高大宽[1] 费舟[1] 冯乐霄 殷安安[1] 董秋峰[1] 陈晓燕[1] 

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2015年第4期297-301,共5页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的研究D-阿洛糖对小鼠脑缺血再灌注损伤后血脑屏障的影响。方法 80只小鼠随机分为假手术组(sham组)、脑缺血模型组(MCAO组)、D-阿洛糖治疗组(D-allose组)、生理盐水对照组(NS组)。采用插线法制备小鼠大脑中动脉栓塞(MCAO)模型,尾静脉注射D-阿洛糖(300 mg/kg),运用改良小鼠神经功能缺损评分(m NSS)对小鼠脑缺血再灌注损伤程度评分;通过1%氯化三苯基四氮唑(TTC)染色,计算脑梗面积百分比;运用干-湿重法,测脑含水率;通过伊文思蓝(EB)含量反映血脑屏障的损伤程度;通过HE染色观察脑缺血再灌注损伤后梗死区的病理变化;采用免疫组化检测基质金属蛋白酶9(MMP-9)的表达。结果与MCAO组相比,D-allose可以明显降低小鼠脑缺血再灌注后神经功能缺损评分,减小小鼠脑梗死面积,减轻脑水肿,降低EB含量,减轻脑组织的病理损伤程度,并且明显减少MMP-9表达(P<0.05)。结论 D-阿洛糖可以保护小鼠脑缺血再灌注后的血脑屏障,其机制可能与抑制MMP-9的表达有关。Objective To study the effect of D-allose on on the blood-brain barrier permeability following focal cerebral ischemia/reperfusion in mice.Methods The focal cerebral ischemia-reperfusion model was induced by the middle cerebral artery occlusion（MCAO）.80 male Balb/c mice were randomly divided into sham group, MCAO group, NS group, D-allose group.With a model of reversible middle cerebral artery occlusion in mice, D-allose was injected in caudal vein（300 mg/kg）.The neurological deficit score was performed by modified neurological severity scores（mNSS）, Infarct volume was calculated by triphenyl tetrazolium chloride （TTC） stain.Brain water content was measured by Dry/Wet method.Blood-brain barrier （BBB） permeability was evaluated by Evans blue extravasation. The histopathological changes was observed by HE staining and the expression of matrix metalloproteinase-9 （MMP-9） was detected by immunohistochemistry method.Results Compared with MCAO group, D-allose group significantly lowered neurological deficit score, reduced the infarct volume, alleviated brain water content markedly, D-allose diminished the extent and quantity of Evans blue extravasation（P〈0.05）.Compared with MCAO group, D-allose decreased the level of MMP-9 expression （P〈0.05）and ameliorated the histopathological changes（P〈0.05）. Conclusion D-allose has the protective effect on the blood-brain barrier following focal cerebral ischemia/reperfusion injury in mice, which is closely related to inhibition of MMP-9 expression.

关 键 词：D-阿洛糖 脑缺血再灌注 血脑屏障 基质金属蛋白酶9 

分 类 号：R743[医药卫生—神经病学与精神病学]