PET成像检测慢性MPTP帕金森病小鼠模型症状前期脑局部葡萄糖代谢  

Detection of brain glucose metabolism in mice with chronic MPTP Parkinson＇s disease by PET imaging

作　　者：许冰[1] 卢昌均[1] 肖成华[2] 高殿帅[3] 单保慈[4] 聂彬彬[4] 

机构地区：[1]柳州市中医院脑病科,广西柳州545001 [2]徐州医学院附属医院神经内科,江苏徐州221002 [3]徐州医学院神经生物学教研室,江苏徐州221004 [4]中国科学院高能物理研究所,北京100000

出　　处：《徐州医学院学报》2015年第7期444-447,共4页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金（81160472）;柳州市科技创新能力与条件建设基金（2013F010401）

摘　　要：目的探讨帕金森病（PD）前期多巴胺能神经元受损至出现局部病理改变前脑部葡萄糖代谢的改变。方法腹腔注射1-甲基-4-苯基-1，2，3，6四氢吡啶（MPTP）制作慢性PD小鼠模型，对照组注射生理盐水。于不同时间点，PET成像检测小鼠脑黑质及纹状体葡萄糖代谢的变化，蛋白电泳检测黑质酪氨酸羟化酶（TH）表达。结果小鼠双侧纹状体出现葡萄糖代谢降低时黑质致密部TH和磷酸化TH表达水平并无明显改变。结论PD小鼠模型双侧纹状体葡萄糖代谢降低早于黑质TH的下降。Objective To investigate the changes of brain glucose metabolism in mice at the early stage of Parkinson＇s disease （PD） when local pathological disorders does not appear. Methods A chronic PD model was established through intraperitoneal injection of MPTP into mice, while control mice were injected with normal saline. The changes of glucose metabolism in the substantia nigra and striatum were detected by PET imaging. The amounts of tyrosine hydroxy-lase （TH） and phosphor - TH （ p - TH） in the substantial nigra were measured by Western blotting. Results There was no remarkable change in the amounts of TH and p - TH in the substantial nigra when the glucose metabolism in the bilateral striatum of mice became weakened. Conclusion The weakening of glucose metabolism in the bilateral striatum appears before the levels of TH and p - TH are reduced in the substantial nigra.

关 键 词：1-甲基-4-苯基-1 2 3 6四氢吡啶 帕金森病 多巴胺能神经元 正电子发射断层扫描 

分 类 号：R74[医药卫生—神经病学与精神病学]