苦参碱通过下调STAT3信号通路抑制骨肉瘤Saos-2细胞增殖的实验研究  

Matrine suppresses the proliferation of osteosarcoma Saos-2 cells by inactivation of STAT3

作　　者：严小明[1] 连福明[1] 林智勤[1] 张维[2] 

机构地区：[1]福建医科大学附属三明第一医院骨科,福建三明365000 [2]成都医学院附属第一医院,四川成都610500

出　　处：《西部医学》2015年第9期1286-1289,共4页Medical Journal of West China

基　　金：四川省教育厅课题(11ZB172)

摘　　要：目的观察苦参碱对成人骨肉瘤Saos-2细胞增殖的调节作用及其与STAT3活化水平的联系。方法将成人骨肉瘤Saos-2细胞分为对照组(Control组)、低剂量苦参碱干预组(MAT-L干预组)及高剂量苦参碱干预组(MATH干预组)3组。使用MTT法对不同时间点(0、24和48h)各组Saos-2细胞活性进行检测;使用qPCR对干预24小时后Saos-2细胞的survivin mRNA水平进行分析,使用western blot法对干预24小时后Saos-2细胞STAT3的活化水平及survivin蛋白表达水平进行分析。结果与对照组相比,给予苦参碱干预的Saos-2细胞活性呈时间依赖性和浓度依赖性下降,差异均有统计学意义(均P<0.05);给予Saos-2细胞不同浓度的苦参碱干预24小时后,survivin mRNA和蛋白的表达水平及STAT3的活化水平呈浓度依赖性降低,差异均有显著统计学意义(均P<0.05)。结论苦参碱可以通过下调STAT3的活化抑制成人骨肉瘤Saos-2细胞survivin的表达,从而抑制肿瘤细胞的增殖。该研究为苦参碱应用于骨肉瘤及其他恶性肿瘤的临床治疗提供了实验基础。Objective To explore the anti-proliferative value of matrine （MAT） in osteosarcoma Saos-2 cells, and its underlying mechanism. Methods Osteosarcoma Saos-2 cells were divided into 3 groups, Control group, Low-dosage matrine （MAT-L） group and High-dosage matrine （MAT-H） group. MTT was performed to measure the cell viabilities of Saos-2 cells in different time points （0h, 24h and 48h）. After 24 hours interventions, the mRNA expression of sur vivin was analyzed by qPCR. Meanwhile, the protein expression of survivin and the activation of STAT3 were detected by western blot. Results The cell viabilities of Saos-2 cells were time-dependently and dosage-dependently inhibited by MAT administrations. Meanwhile, after 24 hours intervention, the mRNA and protein expression of survivin, and the activation of STAT3 were all dosage-dependently down-regulated by MAT in Saos-2 cells. Conclusion Matrine （MAT） could inhibit the tumor proliferation and survivin expression through inactivation of STAT3 in osteosarcoma Saos-2 cells.

关 键 词：SAOS-2细胞 苦参碱 STAT3 SURVIVIN 

分 类 号：R-33[医药卫生]