大鼠卡氏肺孢子菌肺炎模型建立及其病理生理学相关细胞因子的变化  

Modeling of pneumocystispneumonia in rats and the change of pathophysiological cytokine

作　　者：孙艳[1] 伊丽孜拉.伊克拉木 高剑[2] 陈永杰[1] 赖星星[1] 刘云飞[1] 

机构地区：[1]新疆医科大学临床医学院 [2]新疆医科大学基础医学院人体寄生虫学教研室,新疆乌鲁木齐830011

出　　处：《西部医学》2015年第9期1290-1294,共5页Medical Journal of West China

基　　金：国家级大学生创新创业训练计划项目(201310760002);新疆医科大学大学生创新性实验计划项目(CX2013002);新疆医科大学博士(后)科研项目启动基金并新疆医科大学科研创新基金(XJ201222)

摘　　要：目的观察大鼠卡氏肺孢子菌肺炎(PCP)模型建立过程中鼠肺组织学、病原学、外周血肿瘤坏死因子-α(TNF-α)、可溶性粘附分子-1(sICAM-1)和白介素-8(IL-8)含量的动态变化。方法建立大鼠PCP模型,观察建模2、4、6、8和10周时大鼠的发病状况、体重、肺重改变、鼠肺组织学变化、鼠肺印片中卡氏肺孢子菌(PC)包囊形态和增殖数量,并检测大鼠外周血血清中TNF-α、sICAM-1和IL-8的含量。结果建模2周时就可在大鼠肺脏印片中发现PC包囊,但建模6周和8周时PC包囊增殖无显著差异(P>0.05)。建模4周时大鼠出现明显的PCP病征和鼠肺组织学变化,大鼠外周血血清中TNF-α、sICAM-1和IL-8的含量也在建模4周时出现升高,其中TNF-α和sICAM-1升高迅猛,IL-8升高缓慢。结论大鼠建模4周后可出现较为明显的PCP相关病征,而建模6周和8周之间可能存在肺孢子菌与宿主相互作用的相持时期。Objective To observe the dynamic changes of pulmonary histology, pathogenic, and content of TNF-α, sICAM-1 and IL-8 in serum in rats during the establishment of pnenmonia model for pnenmocystis carinii. Methods The pnenmonia model for pnenmocystis carinii in rats was established. The changes of rat weight, lung weight and lung his- tology and morphology and number of pnenmocystis carinii cysts in rats 2 weeks, 4 weeks, 6 weeks, 8 weeks, and 10 weeks after establishment were observed. TNF-α, sICAM-1 and IL-8 in serum in rats were measured. Results The cysts of pnenmocystis carinii were found in lung printing of rats as early as 2 weeks after PCP model established, but no significant differences of proliferation of PC cysts were found between 6 weeks and 8 weeks after PCP model established 6 weeks and 8 weeks. The symptoms and histological changes of PCP obviously appeared 4 weeks after PCP model established. The content of TNF-α, sICAM-1 and IL-8 in serum in rats also significantly increased 4 weeks after PCP model established, while the content of TNF-α and sICAM-1 increased rapidly compared that of IL-8. Conclusion All PCP-related symptoms can be observed 4 weeks after PCP model established, and a stalemate stage of pneumocystis-host interaction may existed between 6 weeks and 8 weeks after PCP model establishment.

关 键 词：卡氏肺孢子菌肺炎 肺孢子菌包囊 组织学变化 肿瘤坏死因子-α 可溶性粘附分子-1 白介素-8 

分 类 号：R-33[医药卫生]