HER-2基因表达在乳腺原发癌和淋巴结转移癌发病中的作用研究  

Role of HER-2 gene expression in pathogensis of breast primary carcinoma and lymph node metastatic carcinoma

作　　者：欧武英[1] 钟威达 

机构地区：[1]海南省海口市人民医院检验科,570208 [2]海南妇产科医院检验科,海口570105

出　　处：《检验医学与临床》2015年第17期2582-2583,2586,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨使用荧光原位杂交技术检测乳腺原发癌和淋巴结转移癌HER-2基因的表达及意义。方法选取海口市人民医院2013年1月至2014年1月收治的乳腺原发癌患者40例作为研究对象,对其临床资料进行回顾性分析。应用荧光原位杂交技术(FISH)与免疫组织化学技术(IHC)对所有患者手术切除标本的HER-2基因表达予以检测。结果 FISH阳性率为42.5%,IHC为57.5%;IHC阴性时与FISH符合率为88.2%,IHC(+)与FISH符合率为37.5%,IHC(++)与FISH符合率为83.3%,IHC(+++)与FISH符合率为100.0%。淋巴结转移阳性率为66.67%,未转移阳性率为32.14%,对比差异有统计学意义(P<0.05)。结论 IHC对HER-2基因予以检测时若结果为阴性或者强阳性则与FISH符合率较高,同时HER-2基因表达与腋淋巴结转移之间呈正相关关系。Objective To investigate the expression and significance of HER‐2 gene expression in primary breast cancer and lymph node metastatic carcinoma by using fluorescence in situ hybridization technique .Methods 40 cases of primary breast cancer in the Haikou Municipal People′s Hospital from January 2013 to January 2014 were selected as the research subjects and their clinical data were retrospectively analyzed .The fluorescence in situ hybrid‐ization （FISH） technique and immunohistochemical （IHC） techniques were used to detect the expression of HER‐2 gene in operation excision specimens of all patients .Results The positive rate of FISH was 42 .5% ,which of IHC was 57 .5% ;in negative IHC ,the coincidence rate with FISH was 88 .2% ,the coincidence rate of IHC （＋ ） with FISH was 37 .5% ,which of IHC （＋ ＋ ） with FISH was 83 .3% and which of IHC （＋ ＋ ＋ ） with FISH was 100% . The lymph node metastasis positive rate was 66 .67% ,non‐lymph node metastasis positive rate was 32 .14% ,the difference was statistically significant（P〈0 .05） .Conclusion Detecting HER‐2 gene by IHC ,if the result is negative or strongly positive ,the coincidence rate with FISH is higher ,at the same time the HER‐2 gene expression is posi‐tivele correlated with axillary lymph node metastasis .

关 键 词：荧光原位杂交技术 乳腺原发癌 淋巴结转移癌 HER-2基因 

分 类 号：R737.9[医药卫生—肿瘤]