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作 者:李伟宏[1] 黄睿[2] 林华[2] 李庆艳[2] 郑新颖[2] 吕俏[2] 高宁[2]
机构地区:[1]浙江大学医学院附属第二医院,浙江杭州310052 [2]四川大学华西口腔医院口腔修复科,四川成都610041
出 处:《上海口腔医学》2015年第4期385-389,共5页Shanghai Journal of Stomatology
基 金:国家自然科学基金(81371182);浙江省自然科学基金(LY13H140005;Y2110400);浙江省卫生厅科技项目(2013KYA124)~~
摘 要:目的 :研究铍离子(Be2+)对牙龈卟啉单胞菌细胞形态和细胞膜离子的影响,探讨镍铬合金对牙周损伤的微生物学机制。方法:用含不同浓度Be2+的培养液厌氧培养牙龈卟啉单胞菌24 h。光镜及扫描电镜下观察不同浓度的Be2+对牙龈卟啉单胞菌细胞形态的影响,同时以X线能谱仪分析细胞膜离子含量的变化。采用SPSS13.0软件包对数据进行统计学分析。结果:Be2+浓度大于2.5 mg/L时,牙龈卟啉单胞菌菌体形态改变。牙龈卟啉单胞菌细胞膜表面Na原子和Ca原子含量随Be2+浓度升高呈降低趋势(P<0.05)。结论:Be2+会改变细菌形态和细胞膜成分,引起细菌功能变化,从而可能导致口腔正常微生态环境失衡,引起牙周疾病。PURPOSE: To evaluate the effect of beryllium(Be2+) on the morphology and chemical elements on cell membrane of Porphyromonas gingivalis(P. gingivalis), thus to explore the microbiologic mechanisms of periodontal diseases. METHODS: P. gingivalis was put into the culture with different Be2+concentrations and anaerobically cultured for 24 hours. The morphologic change of P. gingivalis was observed under microscope and scanning electronic microscope(SEM), and chemical elements of cell membrane were observed by X-ray energy dispersion spectrum(EDS). The data was statistically analyzed with SPSS13.0 software package. RESULTS: The morphology of P.gingivalis altered obviously at the concentration greater than 2.5 mg/L, which was manifested by the sharpness of border and depression on the surface. With the increased concentration of beryllium, the Na and Ca peak descended on the surface of P. gingivalis.CONCLUSIONS: Beryllium can interfere with the morphology of P. gingivalis, and lead to the changes of chemical elements on cell membrane of P. gingivalis, which may result in a disturbance in the microecologic balance of subgingival microbes and eventually contribute to periodontal diseases.
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