ffh基因沉默对变形链球菌耐氟菌株耐酸性的影响  

Evaluation of the effect for ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans

作　　者：李振玲[1] 张志民[1] 李朋莲[1] 张桐菲[1] 李天博[1] 刘富萍[1] 

机构地区：[1]吉林大学口腔医院牙体牙髓病科,吉林长春130021

出　　处：《上海口腔医学》2015年第4期395-399,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(3A411X463431)~~

摘　　要：目的 ：分析ffh基因沉默对变形链球菌耐氟菌株体外耐酸能力的影响。方法 ：利用电穿孔法,将si RNA转入UA159-FR,使其与ffh基因靶向位点结合,筛选出含ffh基因沉默的变形链球菌耐氟菌株,与UA159-FR的标准菌液分别在不同p H值（以0.5为间隔,p H为3.5~7.5）的BHI液体培养基中37℃微需氧（95%N2、5%CO2）培养24 h,离心,采用p H计测定培养物上清的终末p H值;5 m L生理盐水稀释细菌沉淀,用紫外分光光度计测定600 nm处的吸光度,采用SPSS 17.0软件包对所得数据进行统计学分析。结果：原菌株UA159-FR与ffh基因沉默的变形链球菌UA159-FR相比,Δp H差异显著（P〈0.05）,且前者高于后者。两者比较p H=3.5~5.0时,OD600有显著差异（P〈0.01）;p H=5.5~7.5时,OD600有显著差异（P〈0.05）,且两者生长趋势相似。结论：ffh基因沉默对变形链球菌耐氟菌株的耐酸性有一定影响。PURPOSE： To analyze the effect of ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans in vitro. METHODS： By using electroporation, UA159-FR was transformed and combined with targeted site of ffh gene sequence, and the best piece of si RNA for fluoride resistant Streptococcus mutans was screened. In different values of p H of BHI, they were cultured for 24 hours with UA159-FR respectively, and then centrifugated to determine the p H and OD600.SPSS17.0 software package was used to analyze the data. RESULTS： The aciduricity of UA159-FR had significant differences compared with ffh gene silencing for UA159-FR in Δp H（P〈0.05）, and the former was higher than the latter. At p H=3.5 ~5.0, P〈0.01; at p H=5.5 ~7.5, P〈0.05. Significant differences were noted in OD600 and their growth tendency were similar. CONCLUSIONS： The aciduricity of fluoride resistant Streptococcus mutans has significant effect when the ffh gene is silenced.

关 键 词：FFH 基因沉默 变形链球菌耐氟菌株 耐酸性 

分 类 号：R781.1[医药卫生—口腔医学]