rhPDGF-BB与rhTGF-β_1联合应用对大鼠正畸牙破骨细胞FAK蛋白表达的影响  

The influence of combined use of rhPDGF-BB and rhTGF-β_1 on protein expression of FAK in osteoclast during orthodontic tooth movement in rats

作　　者：张疆弢[1] 梅梅[1] 江策[1] 丰雷[1] 黄瑾[1] 王家佳[1] 

机构地区：[1]遵义医学院附属口腔医院正畸科,贵州遵义563003

出　　处：《上海口腔医学》2015年第4期423-427,共5页Shanghai Journal of Stomatology

基　　金：贵州省科学技术基金[黔科合J字LKZ(2011)05号]~~

摘　　要：目的:探讨重组人血小板衍生生长因子BB(rhPDGF-BB)与转化生长因子β1(rhTGF-β1)联合应用对大鼠正畸牙压力侧破骨细胞FAK蛋白的表达变化。方法:160只SD大鼠随机分为实验组(A)和对照组(B)。每组根据检测时间点不同分为5个亚组,分别建立正畸牙移动模型。A组从加力当天开始,隔天注射rhPDGF-BB与rhTGF-β1混合液0.1 m L。加力后1、4、7、10、14 d,分别处死每组中1亚组大鼠。体式显微镜测量牙移动距离的改变,TRAP染色观察压力侧破骨细胞数量变化,免疫组织化学染色法检测FAK蛋白水平的表达变化。采用SPSS17.0软件包对数据进行统计学分析。结果:A组牙移动距离均大于B组,除加力第1天无显著差异(P>0.05)外,其余各时间点差异均具有显著性(P<0.05);A组破骨细胞数目增加较B组明显,除第14天无显著差异外,其余各时间点均具有显著差异(P<0.05);A组破骨细胞内FAK蛋白表达明显高于B组,任何时间点相比,均具有显著差异(P<0.05)。结论:rhPDGF-BB和rhTGF-β1的联合协同作用上调了正畸牙压力侧破骨细胞内FAK蛋白的表达,进一步促进破骨细胞的分化、增殖及骨吸收,从而加速正畸牙的移动速率。PURPOSE： To explore the combined effects of recombinant human platelet-derived growth factor-BB（rhPDGF-BB）and recombinant human transforming growth factor-β1（rhTGF-β1） on protein expression of FAK in osteolasts of the pressure side of orthodontic tooth in rats. METHODS： One hundred and sixty male Sprague-Dawley rats were randomly divided into 2 groups： experimental group（A） and control group（B）. Each group was subdivided equally into5 subgroups. Orthodontic tooth movement model was established. The experimental group received injection of 0.1 m L solution, containing 10 ng rhPDGF-BB and 5 ng rhTGF-β1every other day from day 1. Rats in each subgroup were sacrificed at each of five time points（1, 4, 7, 10 and 14 days） after appliance placement. The distances of the teeth movement were measured using stereomicroscope, and the changes of the amount of osteoclasts were detected by using tartrate resistant acid phosphatase histochemistry（TRAP）. The protein expressions of FAK were assayed by immunohistochemical technique. SPSS17.0 software package was used for statistics analysis. RESULTS： Teeth in group A moved more rapidly than group B, and there were significant differences between 2 groups（ P〈0.05） except subgroup A1 and B1（P 〉0.05）. The number of osteoclasts in group A was significantly higher than in group B. Except at 14 days,significant differences existed in between other time points（P 〈0.05）. The protein expression of FAK in group A was significantly higher than in group B at any time points. CONCLUSIONS： The combined effects of rhPDGF-BB and rhTGF-β1up-regulate the protein expression of FAK within osteoclast in the pressure side, further promoted osteoclast differentiation, proliferation and bone resorption, thus accelerate the rate of orthodontic tooth movement.

关 键 词：血小板衍生生长因子BB 转化生长因子Β1 正畸牙 破骨细胞 FAK 

分 类 号：R783.5[医药卫生—口腔医学]