纳米银抗菌剂的骨内相容性研究  

Intraosseous biocompactability of silver nanoparticles

作　　者：王梓屹[1] 高燕[2] 胡晓莉[2] 庄沛林[2] 蒋晓琼[2] 马可[2] 

机构地区：[1]广州医科大学附属第二医院口腔科,510260 [2]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州2510055

出　　处：《中华口腔医学研究杂志（电子版）》2015年第4期17-21,共5页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省科技计划(2010B031100023)

摘　　要：目的观察纳米银抗菌剂植入豚鼠下颌骨后产生的组织反应,评价其组织相容性。方法选取豚鼠20只,在其双侧下颌骨分别植入载有0.02%纳米银凝胶和氢氧化钙糊剂的铁氟龙载体,术后4及12周各处死10只动物,解剖含植入物的下颌骨块并行组织学切片染色,光学显微镜下观察记录炎症反应程度,并以Kruskal-Wallis非参数检验分析结果。结果 4周时,0.02%纳米银凝胶组炎症计分3例为0分、7例为1分,镜下见少量淋巴细胞、多核巨细胞等慢性炎症细胞浸润,且可见类骨质形成;氢氧化钙组5例为1分、5例为2分,可见糊剂被部分吸收,炎性肉芽组织进入载体腔内。两组间炎症计分差异有统计学意义(Z=2.805,P=0.005)。12周时,0.02%纳米银凝胶组计分8例为0分、2例为1分,可见载体开口处被薄层纤维结缔组织包绕,少有炎症细胞浸润;氢氧化钙组6例为0分、4例为1分,可见成熟肉芽组织长入载体内部,骨组织无或轻度炎症浸润。两组间炎症计分差异无统计学意义(Z=0.951,P=0.342)。结论纳米银抗菌剂在豚鼠颌骨内组织相容性良好,且具有促进骨组织生长修复的潜能,将其应用于根管消毒有望提高根管治疗成功率。Objective The aim of this study was to evaluate the intraosseous biocompatibility of silver nanoparticles implanted in mandibular bone of guinea pigs. Methods Two teflon carriers loaded with 0.02% silver nanoparticle gel and calcium hydroxide paste were respectively implanted into either side of the mandibles of 20 guinea-pigs. After 4 and 12 weeks, guinea-pigs were killed, and the specimens were prepared for routine histological examination. Inflammation responses were evaluated by light microscope. The results were analyzed by Kruskal-Wallis nonparametric test. Results After 4 weeks, the inflammation scores of 3 samples were 0, and the other 7 samples were 1 in 0.02%silver nanoparticle gel group. Chronic inflammatory cells such as lymphocytes and polykaryocytes were found under light microscopy. The scores of 5 samples in the group of calcium hydroxide were 1, andthe other 5 samples were 2. The root canal paste were partly absorbed, and granulation tissues grew into the carriers. A significantly statistical difference was found between the scores of the two groups （Z=2.805,P=0.005）. After 12 weeks, the scores of 8 samples in silver nanopartical group were 0, and the other 2 samples were 1. In calcium hydroxide group, 6 samples were scored 0, and the other 4 samples were scored 1. Both was no significant difference （Z=0.951,P=0.342）. Conclusion Silver nanoparticle shows good biocompatibility in bone tissue and should be further evaluated as an intra-canal medicament during root canal therapy.

关 键 词：纳米银 氢氧化钙 豚鼠 生物相容性 

分 类 号：R782.2[医药卫生—口腔医学]