c-myb和PTEN在结直肠癌变过程中的差异表达  

Differential Expression of C-myb and PTEN in Tumor Development and Progression of Colorectal Carcinoma

作　　者：王晓晓[1] 白莲梅 马玉珍 任宇 仓明[5] 

机构地区：[1]包头医学院 [2]内蒙古自治区人民医院消化内科,内蒙古呼和浩特市010017 [3]内蒙古自治区人民医院生殖中心,内蒙古呼和浩特市010017 [4]内蒙古自治区人民医院临床医学研究中心,内蒙古呼和浩特市010017 [5]内蒙古大学哺乳动物繁殖生物学与生物技术重点实验室,内蒙古呼和浩特010021

出　　处：《内蒙古医学杂志》2015年第7期774-777,F0002,共5页Inner Mongolia Medical Journal

基　　金：内蒙古自治区自然科学基金项目(项目编号:2013MS111222)

摘　　要：目的研究c-myb和PETN在结直肠正常组织、腺瘤组织和癌组织中的表达及其临床意义。方法收集结直肠正常组织、腺瘤组织和癌组织各30例,分别利用实时定量PCR和Western-Blot检测c-myb和PETN在不同组织中mRNA和蛋白的表达量并比较其差异性。利用免疫组化法确定c-myb和PETN在不同组织的位置。结果癌组织中c-myb mRNA和蛋白表达分别是腺瘤组织中c-myb的1.31倍和2.30倍且差异显著;癌组织中PTEN mRNA表达是腺瘤组织中PTEN的1.38倍且差异显著,但PTEN蛋白表达在这两种组织中却无明显差异。结论 c-myb和PTEN的表达可能与结直肠癌发生发展有密切的关系,尤其对早期癌的检测具有重要意义。Objective To study the expression of c-myb and PTEN and their clinical significances in colorectal normal tissues,adenoma tissues and carcinoma tissues.Methods Real-time quantitative PCR and western-Blotting were used in detection of c-myb and PTEN in normal colorectal tissues,adenomas and carcinomas（30cases）and compared their differences.Immunohistochemistry was used to determine the location of c-myb and PETN in different tissues.Results C-myb mRNA and protein expression of tissues from adenoma tissues were 1.31 and 2.30 greater than that of tissues derived from carcinoma tissues,respectively.PTEN mRNA expression of tissues from adenomas were 1.38 greater than that of tissues derived from carcinomas.But PTEN protein expression was no significant difference in adenoma tissues and carcinoma tissues.Conclusion Expression of c-myb and PTEN may be closely related with the occurrence and development of colorectal cancer,especially in detecting early cancer has important significances.

关 键 词：C-MYB PETN 差异表达 结直肠癌 

分 类 号：R735[医药卫生—肿瘤]