微卫星标记在布氏田鼠封闭群遗传结构研究中的应用  

Applications of microsatellite marker technology in the genetic structure research for closed colony of Brandt's voles

作　　者：张曼[1] 施海霞[1] 宋铭晶[1] 

机构地区：[1]中国医学科学院医学实验动物研究所北京协和医学院比较医学中心卫生部人类疾病比较医学重点实验室,北京100021

出　　处：《中国比较医学杂志》2015年第7期34-38,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金项目(31301890);北京市自然科学基金资助项目(7122110)

摘　　要：目的使用微卫星标记分析连续三代布氏田鼠封闭群遗传结构的稳定性。方法使用高盐沉淀法从鼠尾中提取布氏田鼠基因组DNA,将筛选出7对微卫星引物并用荧光标记(Fam),然后对布氏田鼠封闭群连续三代的基因组DNA进行PCR扩增,测序仪检测PCR产物,整理原始数据并对布氏田鼠基因组DNA进行微卫星分析。结果由平均有效杂合度和多态信息含量的分析得出该布氏田鼠种群传代过程中保持着较高而且稳定的杂合度。结论该实验结果说明本实验室布氏田鼠封闭群的遗传结构比较稳定。Objective Analysis of the genetic structure stability of Brandt ’ s vole （ lasiopodomys brandtii ） in closed colony using microsatellite marker technology.Methods Genomic DNA was extracted from tail tip using high-concentration-salt precipitation methods.Marked with fluorescent tags（ Fam） , 7 microsatellite primers were filtered out by PCR, and the DNA structure of three consecutive generations of Brandt’ s vole was analyzed by microsatellite marker. Results By the analysis of the average heterozygosity and polymorphism information content, Brandt’ s vole populations maintaineda closed group of qualified genetic structure.Conclusions The present results show that the closed group of Brandt’ s vole species in our laboratory maintain a stable genetic structure.

关 键 词：布氏田鼠 封闭群 遗传结构 微卫星DNA标记 

分 类 号：R-332[医药卫生]