OLK患者与OSCC癌患者的原癌基因蛋白差异及相关性研究  

Correlation and differences in proto oncogene protein of patients with OLK and OSCC

作　　者：李崴嵬[1] 魏琦玲 杨金晖[1] 佟静[1] 崔秋萍[1] 

机构地区：[1]河北省唐山工人医院口腔科,河北唐山063000

出　　处：《中国生化药物杂志》2015年第6期65-67,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：2012年唐山市科技局计划项目(121302119a)

摘　　要：目的探讨原癌基因蛋白在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)及口腔白斑中(oral leukoplakia,OLK)的意义。方法选取唐山工人医院口腔科已确诊为口腔白斑的患者40例作为OLK组,确诊为口腔鳞状细胞癌患者40例作为OSCC组,同时期体检正常志愿者40例作为对照组,取病变组织、正常口腔粘膜作为样本,研磨后取上清液,采用酶联免疫法检测3组研究对象样本中MDM2蛋白,p53蛋白,Ki-67蛋白的含量情况,并分析3者的相关性。结果 3组间MDM2、p53表达水平比较均为OSCC组>OLK组>对照组;3组间Ki-67表达水平比较,OSCC组>OLK组>对照组(P<0.05);MDM2,p53,Ki-67在口腔白斑及口腔鳞状细胞癌中表达呈正相关(P<0.05),在正常粘膜中表达无相关。结论口腔白斑发展成为口腔鳞状细胞癌与MDM2,p53,Ki-67的高表达有关,MDM2、p53、Ki-67 3者之间呈正相关。Objective To analyse the significance of proto oncogene protein in oral squamous cell carcinoma and oral leukoplakia.Methods 40 patients who were diagnosed with oral leukoplakia （OLK）,40 patients who were diagnosed with oral squamous cell carcinoma（OSCC）,and at the same time, 40 healthy volunteers as control group were collected.the lesions and normal oral mucosa were taken as samples, left supernatant after grinding, MDM2 protein, p53 protein and Ki-67 protein were detected by ELISA in three groups,and analyse the correlation between three groups.Results Among three groups,MDM2, p53 expression：OSCC group〉OLK group〉control group （P〈0.05）.Ki-67 expression level among three groups, OSCC group〉OLK group〉control group （P〈0.05）.MDM2, p53, Ki-67 expression had a positive correlation in OSCC group and OLK group （P〈0.05）, but there was no obvious correlation in control group.Conclusion When oral leukoplakia developes into oral squamous cell carcinoma, MDM2, p53, Ki-67 shows high expression, and there is a positive correlation between MDM2, p53, Ki-67.

关 键 词：口腔白斑 口腔鳞状细胞癌 MDM2 p53 KI-67 

分 类 号：R739.8[医药卫生—肿瘤]