梵净山水菖蒲总黄酮的提取工艺优化及其抗氧化活性  

Extraction Process Optimization and Antioxidant Activity of Total Flavonoids from Acorus calamus in Fanjing Mountain

作　　者：杨詹詹[1,2] 鲁道旺[1,2] 陈仕学[1,2] 郑静[1] 张立师[1] 

机构地区：[1]铜仁学院材料与化学工程学院,贵州铜仁554300 [2]梵净山特色动植物资源重点实验室,贵州铜仁554300

出　　处：《贵州农业科学》2015年第8期237-240,245,共5页Guizhou Agricultural Sciences

基　　金：贵州省高校重点支持学科项目"野生动植物保护与利用"[黔教合重点支持学科字(2011)232];铜仁市科技局项目"梵净山水菖蒲炮制前后成分含量变化与药理活性研究"[铜市科研(2013)9-4]

摘　　要：为给梵净山水菖蒲的合理开发和科学利用提供参考,采用乙醇加热回流提取法研究提取梵净山水菖蒲总黄酮的工艺条件,采用紫外分光光度法测定提取物对DPPH自由基和羟基自由基的清除率。结果表明:最佳提取工艺为乙醇浓度90%、提取2h、提取温度80℃、料液比1∶20。在该提取条件下,水菖蒲总黄酮的含量为40.83mg/g,提取液对DPPH自由基和羟基自由基具有一定的清除能力,当提取液总黄酮浓度为0.5mg/mL时,其DPPH自由基和羟基自由基的清除率分别达72.46%和73.48%。To provide the references for rational development and scientific utilization of A. calamus in Fanjing Mountain, the technological conditions of extracting total flavonoids from A. calamus in Fanjing Mountain were studied by ethanol refluxed back-flow, the antioxidant activity of the extract was evaluated by ultraviolet speetrophotometry on its ability of scavenging hydroxyl radical and DPPH radical. The results showed that the optimum procedure for ethanol refluxed back-flow of total flavonoids were ethanol concentration 90%, extracting time 2 h, extraction temperature 80℃ solid-liquid ratio 1 ： 20, under the optimum conditions, the content was up to 40. 83 mg/g and the extracting solution has certain antioxidative effect. When the concentration of total flavonoid was 0. 5 mg/mL, the rate of scavenging DPPH radical and hydroxyl radical reached 72.46 G and 73.48 %.

关 键 词：水菖蒲 总黄酮 提取工艺 抗氧化活性 梵净山 贵州 

分 类 号：R284.2[医药卫生—中药学] S567[医药卫生—中医学]