陆川猪HSP27基因的克隆与序列分析  

Cloning and sequence analysis of heat shock protein 27( HSP 27) gene in Luchuan pig

作　　者：黄建芳[1] 吴敏[1] 严雪瑜[1] 吴延军[1] 郭晓萍[1] 蒋钦杨[1] 郭亚芬[1] 兰干球[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530004

出　　处：《黑龙江畜牧兽医》2015年第9期120-123,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家现代农业产业技术体系广西生猪创新团队项目(nycytxgxcxtd-03-15)

摘　　要：为了研究陆川猪HSP27基因的遗传变异情况,试验采用RT-PCR和克隆方法,扩增了HSP27基因的编码区序列,并进行了测序和序列分析及其蛋白质结构预测。结果表明:陆川猪HSP27基因编码区全长624 bp,编码207个氨基酸;与Gen Bank中已发表的猪HSP27基因序列相比,核苷酸有1个位点发生碱基突变,但并未引起相应氨基酸的改变;陆川猪HSP27成熟肽包含α螺旋、β转角、延长线和无规卷曲;陆川猪HSP27基因与野猪的亲缘关系最近。To study the genetic variation of the heat shock protein 27 （ HSP27 ） gene in Luchuan pig, the coding region sequence of HSP27 gene in Luchuan pig was amplified using RT - PCR and a cloning method, and then the sequencing, sequence analysis and the prediction of protein structure of HSP27 were carried out. The results showed that the full - length of the coding region of the HSF27 gene in Luchuan pig was 624 bp, encoding for a protein of 207 amino acids. Compared with the HSP27 gene sequences pubiished in GenBank, a base mutation was detected at one nucleotide site, but it did not cause the change in the corresponding amino acid. The HSP27 mature peptide in Luchuan pig contained α helix, β comer,extension cords and random coils. The Luchuan pig had the closest relationship with Sus scrofa in the HSP27 gene.

关 键 词：陆川猪 HSP27基因 克隆 编码区 序列分析 蛋白质二级结构 结构预测 同源性 

分 类 号：S813.1[农业科学—畜牧学] S828[农业科学—畜牧兽医]