实时荧光定量PCR检测CD44v2在膀胱及尿路上皮癌中的表达  

The detection of CD44v2 expression by using real-time fluorescent quantitative PCR in bladder and urothelial carcinoma

作　　者：刘小荣 王永翔 赵立明 赵小东 刘学军 任玉林 李少君 

机构地区：[1]甘肃省第二人民医院检验科,甘肃兰州730050 [2]甘肃省第二人民医院泌尿外科,甘肃兰州730050

出　　处：《国际检验医学杂志》2015年第17期2478-2479,共2页International Journal of Laboratory Medicine

基　　金：兰州市科技计划资助项目(2012-1-3)

摘　　要：目的探讨CD44变异体(CD44v)2在膀胱及尿路上皮癌中的表达及其在膀胱及尿路上皮癌诊断中的价值。方法选取膀胱及尿路上皮癌患者70例,通过实时荧光定量聚合酶链反应(PCR)检测,从病理分期和临床分期两个方面对患者癌组织标本进行CD44v2表达情况的分析。另外,收集20例正常膀胱黏膜组织标本作为对照。结果 CD44v2在正常膀胱黏膜上皮无阳性表达,拷贝数均小于1×102 copies/mL。在70例膀胱及尿路上皮癌组织中的阳性表达率为42.9%(30/70),Ct值均小于35,基因拷贝数均大于1×104 copies/mL。CD44v2的表达在膀胱及尿路上皮癌不同TNM分期和病理分级间比较,差异有统计学意义(P<0.05)。结论 CD44v2有助于膀胱及尿路上皮癌的早期评估。Objective To investigate the expression of CD44 varant 2（CD44v2） in bladder and urothelial carcinoma ,and to study its significance in the diagnosis of human bladder and urothelial carcinoma .Methods Real‐time fluorescent quantitative PCR was used to analyze the expression of CD44v2 protein in 70 bladder and urothelial carcinoma tissue samples from patients in different pathological and clinical stages .Meanwhile ,20 tissue samples of normal bladder mucosa were collected as controls .Results CD44v2 expression was negative in normal bladder and urothelial mucosa ,the gene copies were less than 1 × 102 copies/mL ,while the posi‐tive expression rate of CD44v2 was 42 .9% （30/70） ,and Ct values were less than 35 and copy number was greater than 1 × 104 cop‐ies/mL .Positive expression rate was correlated with high pathological grades and TNM stages .Conclusion CD44v2 could be an useful indicator for the early assessment of bladder and urothelial carcinoma .

关 键 词：膀胱及尿路癌 实时荧光定量PCR CD44变异体 

分 类 号：R737[医药卫生—肿瘤] R730.43[医药卫生—临床医学]