长链非编码RNA UCA1对胰腺癌细胞系侵袭转移的影响  

Effect of long non-coding RNA UCA1on invasion and metastasis of pancreatic cancer cell lines

作　　者：张尤历[1] 赵义[1] 张行行[1] 王国英[1] 龚爱华[2] 倪鑫[1] 徐岷[1] 

机构地区：[1]江苏大学附属医院消化内科 [2]江苏大学医学院细胞生物学教研室,江苏镇江212000

出　　处：《基础医学与临床》2015年第9期1223-1227,共5页Basic and Clinical Medicine

基　　金：江苏省卫生厅科研项目(H201434);镇江市科技支撑计划(SH2014024)

摘　　要：目的探讨尿路上皮癌相关1(UCA1)在胰腺癌中的表达及其对胰腺癌细胞侵袭转移的影响。方法用荧光定量PCR检测11例胰腺癌组织和配对的癌旁组织及5种胰腺癌细胞系中UCA1的表达,通过RNA干扰技术降低胰腺癌细胞系Bx PC-3的UCA1水平,用Transwell侵袭实验和划痕实验观察Bx PC-3细胞的侵袭和迁移能力,Western blot检测MMP-2和MMP-9的蛋白水平。结果胰腺癌组织的UCA1水平高于相应的癌旁组织(P<0.05),UCA1差异性表达于5种胰腺癌细胞系;干扰UCA1后,Bx PC-3细胞的MMP-2和MMP-9的蛋白水平均降低(P<0.01),侵袭能力和迁移能力明显减弱(P<0.01)。结论 UCA1在胰腺癌中高表达,下调UCA1通过降低MMP-2和MMP-9的表达减弱胰腺癌细胞系Bx PC-3的体外侵袭转移能力。Objective To explore the expression of urothelial carcinoma-associated 1 （ UCA1 ） in pancreatic cancer cell lines and its influence on the invasion and metastasis of the pancreatic cancer cells. Methods The expression of UCA1 in pancreatic cancer tissues and paired adjacent normal tissues （ 11 cases） and 5 pancreatic cancer cell lines was analyzed by real-time PCR. The level of UCA1 in BxPC-3 was knocked down by small interfering RNA. The ability of invasion and migration in vitro of transfected BxPC-3 was detected by Transwell invasion assay and wound healing assay. The protein levels of MMP-2 and MMP-9 were measured by Western blot experiment. Results The expression level of UCA1 in pancreatic cancer tissues was higher than that in paired adjacent normal tissues, and UCA1 differentially expressed in 5 pancreatic cancer cell lines. Down-regulation of UCA1 by siRNA suppressed the expression of MMP-2 and MMP-9 in BxPC3, and dramatically impaired the ability of invasion and migration of BxPC-3. Conclusions UCA1 is over-expressed in pancreatic cancer, and down-regulation of UCA1 attenuates the capacity of invasion and metastasis in vitro of BxPC-3 by decreasing MMP-2 and MMP-9.

关 键 词：胰腺肿瘤 肿瘤侵袭 肿瘤转移 长链非编码RNA 尿路上皮癌相关1 

分 类 号：R735.9[医药卫生—肿瘤]