大豆籽粒氨基酸含量的QTL分析  

QTL Mapping of Seed Amino Acids Content in Soybean

作　　者：陈强[1] 闫龙[1] 雷亚坤 张颖君[1] 邸锐[1] 刘兵强[1] 杨春燕[1] 张孟臣[1] 

机构地区：[1]河北省农林科学院粮油作物研究所/国家大豆改良中心石家庄分中心/农业部黄淮海大豆生物学与遗传育种重点实验室/河北省遗传育种重点实验室,河北石家庄050035

出　　处：《大豆科学》2015年第4期597-600,629,共5页Soybean Science

基　　金：国家现代农业产业技术体系(CARS-004-PS06);国家高技术研究发展计划"863计划"(2012AA101106);河北省科技支撑计划(14226309D)

摘　　要：以＂冀豆12×黑豆＂构建的包含186个家系的F8代重组自交系（RIL）群体为材料,分析16种氨基酸含量,采用性状-标记间单向因素方差分析法（ANOVA）、复合区间作图法（CIM）和完备区间作图法（ICIM）对各氨基酸含量的相关QTL进行检测。结果表明：群体中各性状表型均呈现连续性正态分布并超亲分离。3种方法共检测到谷氨酸、精氨酸、丝氨酸、丙氨酸、苯丙氨酸、异亮氨酸、天冬氨酸和赖氨酸含量相关的14个QTL,分布于B1、D1a、D1b、H、I、J和K连锁群中,贡献率为4.91%~17.02%。其中完备区间作图法检测到的苯丙氨酸含量相关QTL q Phe-20-1贡献率最大为17.02%,增效基因来自于黑豆,并且在该标记区间内利用复合区间作图法同时检测到与天冬氨酸、谷氨酸和赖氨酸含量相关的QTL,分别为q Asp-20、q Glu-20、q Lys-20。位于B1连锁群中精氨酸含量相关的QTL q Arg-11在性状-标记间单向方差分析和ICIM两方法中同时检测到,在ICIM中检测到其贡献率为11.79%,增效基因来自于冀豆12。A cross of Jidou 12 and Heidou was made to construct a recombinant inbred line of F8 population which has 186 progenies as the experimental material. Correlation between 16 amino acid content were analysis in Shijiazhuang environment.ANOVA,CIM and ICIM were used to identify quantitative trait loci. The results showed that the distribution of 16 traits was continuous and normal distribution. Fourteen QTL were detected across three method about glutamic acid,arginine,serine,alanine,phenylalanine,isoleucine,aspartic acid and lysine,distributed at B1,D1 a,D1b,H,I,J and K linkage group,contribution rate from 4. 91% to 17. 02%. q Phe-20-1 had the highest contribution rate（ 17. 02%）,detected by ICIM,favorable alleles originate from Heidou. There were three QTL q Asp-20,q Glu-20 and q Lys-20 detected by CIM in the same marker interval with q Phe-20-1. q Arg-11 was detected by ANOVA and ICIM,contribution rate was 11. 79%,favorable alleles originate from Jidou 12. These results provide a theoretical basis for marker-assisted breeding and fine mapping.

关 键 词：大豆 重组自交系 氨基酸含量 QTL 

分 类 号：S565.1[农业科学—作物学]