地西他滨对Jurkat细胞增殖、凋亡及TAL1基因表达的影响  

The Effect of Decitabine on Proliferiation,Apoptosis and TAL1 Gene Expression in Jurkat Cells

作　　者：王怡[1] 孙玲[1] 王芳[1] 桑丽娜[1] 韩春艳[1] 

机构地区：[1]郑州大学第一附属医院血液科,河南郑州450052

出　　处：《医学与哲学（B）》2015年第8期79-82,共4页Medicine & Philosophy(B)

摘　　要：本研究旨在探讨地西他滨(DAC)对Jurkat细胞增殖、凋亡及对TAL1基因表达的影响。将不同浓度DAC作用于Jurkat细胞,采用WST-1测细胞增殖抑制率,荧光显微镜下观察细胞形态,流式细胞术测细胞凋亡,qPCR检测TAL1mRNA表达。DAC作用于Jurkat细胞24h、48h、72h,24h与48h组比较,差异有统计学意义(P<0.05),而48h组与72h组比较,差异无统计学意义(P>0.05)。5μmol/L DAC作用于Jurkat细胞48h,荧光显微镜下观察细胞核裂解情况。AnnexinV-FITC/PI双标法显示0μmol/L和5μmol/L DAC作用Jurkat细胞48h,实验组凋亡率增高,差异有统计学意义(P<0.01)。qPCR检测TAL1mRNA表达:实验组较对照组下降。DAC呈时间、浓度依赖性抑制Jurkat细胞增殖,并能通过下调TAL1基因的表达诱导细胞凋亡。This study was designed to investigate the decitabine （DAC） on Jurkat proliferation, apoptosis and the expression of the gene for TALl. The effect of different concentrations of DAC in Jurkat, measured using the WST-1 cell proliferation rate, ceil morphology was observed under a fluorescence microscope, apoptosis was detected by flow cytometry, real-time quantitative PCR （qPCR） was to detect TALl mRNA expression. DAC acting on Jurkat 24h, 48h, 72h, 24h and 48h group, the difference was statistically significant （P〈0.05）, and 48h and 72h group, the difference was not statistically significant （P^0. 05）. 5t^mol/L DAC effect on Jurkat cells 48h, nuclei lysis was observed under a fluorescence microscope. AnnexinV-FITC/PI double staining shows 0μmol/L and 5μmol/L DAC role Jurkat 48h, apoptosis rate increased in the experimental group, the difference was statistically significant （P〈0. 01）. qPCR TALl mRNA detect the expression.- the experimental group than the control group decreased. DAC was time, concentration- dependent inhibition of Jurkat cell proliferation and can decrease the expression of genes induced apoptosis TALl.

关 键 词：地西他滨 JURKAT细胞 凋亡 

分 类 号：R733.71[医药卫生—肿瘤]