植入性门静脉主干癌栓兔模型建立及评估  

Implanted main portal venous tumor thrombus model in experimental rabbits: its establishment and evaluation

作　　者：张雯[1] 李说[1] 颜志平[1] 罗剑钧[1] 刘清欣[1] 瞿旭东[1] 王建华[1] 

机构地区：[1]复旦大学附属中山医院介入治疗科,上海200032

出　　处：《介入放射学杂志》2015年第9期801-806,共6页Journal of Interventional Radiology

摘　　要：目的探讨建立稳定的门静脉主干癌栓（MPTT）动物模型并作客观评估，为进一步临床治疗研究提供基础。方法24只新西兰大白兔随机分成对照组（A组，n=10）和实验组（B组，n=14）。实验组在门静脉主干前壁缝一荷包，将瘤条经荷包中央注入门静脉腔内，并通过预留缝线悬挂固定在门静脉主干内壁：对照组开腹后仅在门静脉主干前壁作荷包缝合。术后观察动物一般情况、体重及生存时间。术后每周行多排螺旋CT检查，观察门静脉癌栓（PVTT）生长及转移。分阶段处死两组部分实验兔作病理检查，观察PVTT体积和转移情况。剩余兔观察生存时间。结果实验组门静脉主干成瘤率达100％。实验组（B9～14号兔）及对照组术后第35天平均体重分别为（1．48±0．19）kg和（2．08±0．17）kg，实验组（B9～14号兔）平均生存时间为（41．7±4．72）d。多排螺旋CT显示PVTT及门静脉阻塞后侧支循环和转移灶，病理学检查证实门静脉管腔内癌栓存在及转移灶。结论成功建立了稳定的可供影像学客观评价的MPVTT动物模型，并证实增强多排螺旋CT对诊断和监测PVTT生长和转移有良好价值，为临床治疗MPVTT研究建立基础。Objective To establish a stable animal model of implanted main portal vein tumor thrombus （MPVTT） in rabbits and to evaluate its usefulness in research so as to provide the basis for clinical treatment. Methods Twenty-four New Zealand white rabbits were randomly divided into group A （control group,n=lO） and group B （study group,n=14）. For the rabbits of the study group, a sac-like pouch was sewed up in the anterior wall of the main portal vein, and then the tumor slice was injected into the portal vein through the pouch and it was hung and fixed on the inner wall of the main portal vein with the help of the reserved suture. For the rabbits of the control group, only a sac-like pouch was sewed up in the anterior wall of the main portal vein after opening the abdomen. After the treatment, the animals were kept under observation on the general condition, body weight and survival time. Postoperative multi-slice spiral CT scan was performed once a week to check the growth of portal vein tumor thrombus and the metastasis. The experimental rabbits were separately sacrificed for pathologic examination, the volume of MPVTY was determined and the metastasis was evaluated. The survival time of the remaining rabbits were analyzed. Results The tumor formation rate of the study group was 100%. The mean body weight of the rabbits of the study group （No.9-No.14 rabbits） and the control group at 35 days after the procedure was （1.48±0.19） kg and （2.08±0.17） kg respectively. The mean survival time of the study group （No.9-No.14 rabbits） was （41.7±4.72） days. Multi-slice spiral CT scan revealed MPVTT, metastasis and collateral circulation due to portal vein obstruction. Pathological examination confirmed the presence of thrombus in the portal vein and metastasis. Conclusion Stable MPVTT in animal models that can be used for imaging evaluation are successfully established. This study proves that muhi-slice spiral CT scan is of great value in diagnosing and monitoring the growth of MPVTT and metasta

关 键 词：VX2细胞株 动物模型 肝细胞癌 门静脉癌栓 多排螺旋CT 

分 类 号：R735.7[医药卫生—肿瘤]