半胱胺锌修饰玻碳电极测定饲料添加剂赖氨酸铜螯合物的螯合率  

Cysteamine-zinc(Ⅱ) complex modified glassy carbon electrode for the determination of the chelating rate of lysine-copper(Ⅱ) in feed additive

作　　者：舒绪刚[1] 吴春丽[1] 陈凤[1] 周萌[1] 李翠金[1] 邱桂雄 吴信[3] 印遇龙[3] 

机构地区：[1]仲恺农业工程学院化学化工学院,广州510225 [2]广州天科生物科技有限公司,广州510689 [3]中国科学院亚热带农业生态研究所,长沙410125

出　　处：《分析试验室》2015年第9期1058-1061,共4页Chinese Journal of Analysis Laboratory

基　　金：广东省科技计划项目(2014A020208131;2013B090900;2013B070704081)资助

摘　　要：采用半胱胺锌（C2H7NS）3·Zn SO4·Zn Cl2修饰玻碳电极,以差分脉冲伏安法（DPV）在0.1 mol/L的KCl溶液中对游离Cu2＋进行了测定。游离Cu2＋浓度与氧化峰电流值在0.01～0.5 mmol/L范围内呈良好线性关系,线性回归方程为I=-0.1005c-1.6×10-5,相关系数为R2=98.63,检出限（S/N=3）为0.1μmol/L。通过加标实验验证修饰电极对Cu2＋含量测定,回收率为95.1%～102.6%,RSD（n=5）在3%以内。考察了干扰金属离子（M g2＋,Mn2＋,Zn2＋）对电极检测Cu2＋无影响,制备的修饰电极对赖氨酸铜螯合物不响应,直接测定赖氨酸铜螯合物螯合率为91.78%和91.93%。In this study, an electrochemical sensor composed of （C2H7Ns）3 - ZnSO4 · ZnC12 -modified glass carbon electrode was constructed for the determination of copper （Ⅱ） ions with differential pulse voltammetry （DPV） in 0. 1 mol/L KC1 solutions. The proposed electrochemical sensor had a good response towards Cu2＋ , and the oxidation peak current exhibited linear relationship with the CuE＋ concentration between O. 01 mmol/L and 0.5 mmol/L. The corresponding linear equation was I = - 0. 1005c - 1. 6 × 10-5, with the correlation coefficient of O. 9863 and the detection limit （S/N = 3） of 0. 1 μmol/L. The standard addition test showed that the recoveries were at the range of 95.1% to 102.6% , with the RSD （n = 5） of less than 3 %. Additionally, some common metal ions （Mg2＋ , Mn2＋ , Zn2＋ ） had no interference on the determination of Cu2＋ , and the chelating rates of lysine-copper （Ⅱ） were calculated to be 91.78 % and 91.93 % respectively for the two samples.

关 键 词：半胱胺锌修饰电极 差分脉冲伏安曲线 螯合率 

分 类 号：TQ46[化学工程—制药化工]