ASPP2在人肝星状细胞活化中的作用  

Effect of ASPP2 overexpression on the activation of hepatic stellate cells

作　　者：常远[1] 林明华[1] 欧阳雅博[1] 王珊珊[1] 寇卜心[1] 刘芳[1] 陈德喜[1] 

机构地区：[1]首都医科大学附属北京佑安医院北京市肝病研究所,100069

出　　处：《北京医学》2015年第9期864-866,I0002,共4页Beijing Medical Journal

基　　金：国家自然科学基金(81272266);首都医科大学基础-临床科研合作基金(15JL-L05)

摘　　要：目的探讨p53凋亡刺激蛋白2(apoptosis-stimulating protein of p53-2,ASPP2)对人肝星状细胞活化的调节作用及机制。方法人永生的肝星状细胞系LX-2在孵箱中培养,转染ASPP2腺病毒和单荧光自噬指示体系(GFP-LC3)质粒,免疫印迹法检测ASPP2、α-平滑肌肌动蛋白(α-SMA)、自噬基因表达相关蛋白(Beclin-1)的表达水平。免疫荧光检测,LX-2细胞共转染ASPP2腺病毒和GFP-LC3质粒后,ASPP2过表达对LX-2细胞自噬的影响,M30染色检测ASPP2过表达对LX-2细胞凋亡的影响。结果转染ASPP2腺病毒后LX-2细胞内ASPP2蛋白表达明显增加;与转染对照空载腺病毒的LX-2细胞相比,转染ASPP2腺病毒的LX-2细胞自噬和α-SMA的表达明显减少,肝星状细胞的活化受到抑制;转染ASPP2腺病毒后LX-2细胞的凋亡水平增加明显。结论 ASPP2过表达在体外具有抑制肝星状细胞活化的作用。Objective To investigate the effect of ASPP2 on the activation of hepatic stellate cells.Methods LX- 2 cells were transfeeted ASPP2 adenovirus and LC3-GFP plasmid,then treated with nutrient medium. Expression of ASPP2, α- SMA and Beclin- 1 were analyzed by western bloting. Autophagy was observed by immunofluorescency. Re- sults The protein expression ofASPP2 markly increased after transfecting ASPP2 Adenovirus in LX-2 cells;the level of autophagy andα-SMA significantly decreased compared with the transfecting GFP-ad group,moreover,ASPP2 overexpres- sion promoted the apoptosis of LX-2 cells. Conclusion Overexpression of ASPP2 can inhibit the activation of LX-2 cells in vitro.

关 键 词：p53凋亡刺激蛋白2 肝星状细胞 自噬 

分 类 号：R575.2[医药卫生—消化系统]