酵母SOD1基因缺失突变体应答刚果红胁迫的转录组学分析（英文）  

Genome-wide Transcriptome Analysis of Yeast SOD1 Deletion Mutant in Response to Congo Red Stress

作　　者：张小华[1] 刘向勇[2] 卞伟华[2] 许聪[2] 武玉永[2] 张昭杰[3] 

机构地区：[1]滨州医学院生物技术教研室,山东烟台264003 [2]滨州医学院细胞生物学教研室,山东烟台264003 [3]美国怀俄明大学动物与生理学教研室,怀俄明拉勒米wy82071

出　　处：《中国生物化学与分子生物学报》2015年第9期960-966,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：Supported by Natural Science Foundation of Shandong Province(No.ZR2012CQ041);Science and Technology Development Plan of Yantai City(No.2013ZH097)~~

摘　　要：SOD1基因编码的铜锌超氧化物歧化酶是酵母细胞中最重要的抗氧化酶.前期研究发现,SOD1基因缺失(sod1Δ)导致酵母细胞对真菌细胞壁抑制剂刚果红(Congo red,CR)的敏感性增加,提示细胞抗氧化能力与细胞壁稳定性相关.本研究采用酵母全基因组表达谱芯片,比较了CR胁迫条件下,野生型酵母细胞和sod1Δ酵母细胞的转录表达谱.结果表明,与野生型酵母细胞相比,sod1Δ酵母细胞中260个基因发生了显著差异表达(140个基因表达上调、120个基因表达下调).随机选取12个差异表达基因采用定量PCR验证,结果与芯片分析结果一致.差异表达基因功能主要涉及细胞壁(几丁质合成)、细胞代谢、细胞防御(抗氧化和热冲击蛋白)、蛋白质合成以及大量功能未知基因.进一步研究发现,CR处理后,细胞壁几丁质含量和细胞内氧化应激指标丙二醛(MDA)含量在sod1Δ酵母细胞中显著升高,而在野生型酵母细胞中无明显变化,与芯片筛选差异表达基因的生物学功能分析结果一致.本研究提供了在全基因组水平上对SOD1基因与细胞壁应激反应之间关联的新认识.The SOD1 gene encodes an anti-oxidative enzyme,Cu / Zn-superoxide dismutase,that is required for the tolerance of cell walls to a perturbing agent Congo red（ CR）. In this study,we used a yeast DNA microarray to compare the genome-wide transcription profiles of sod1Δ and the wild type in response to CR stress. The results showed that 260 genes in sod1Δ（ 140 up-regulated and 120 downregulated） were differentially expressed from the wild type yeast. Twelve genes were randomly selected and verified by q PCR. The differentially expressed genes were mainly related to chitin biosynthesis,cell metabolism,cell defence,protein synthesis. Some genes were with unknown functions. CR treatment caused a significant increase of the content of cell wall chitin and oxidative damage shown in malondialdehyde（ MDA） levels in sod1Δ yeast. This study provides useful data about the effect of yeast SOD1 in cell wall stress response at the genome level.

关 键 词：铜锌超氧化物歧化酶 细胞壁 DNA芯片 氧化胁迫 酵母 

分 类 号：Q93[生物学—微生物学]