鱼藤酮诱导PC12细胞自噬的初步研究  

Preliminary Study of Autophagy Induced by Rotenone in PC12 Cells

作　　者：刘腾[1] 黄永秀[1] 王丹[2] 崔涌泉[3] 王建东[2] 张红[1] 

机构地区：[1]成都医学院检验医学院,成都610500 [2]成都医学院生物医学系,成都610500 [3]中国人民解放军第37医院检验病理科,雅安625000

出　　处：《成都医学院学报》2015年第4期415-419,共5页Journal of Chengdu Medical College

基　　金：四川省教育厅基金项目(No:13ZB0094);国家级大学生创新训练项目(No:201313705010);发育与再生四川省重点实验室基金项目(No:SYS12-004)

摘　　要：目的研究鱼藤酮处理对PC12细胞自噬水平的影响。方法培养PC12细胞,分别用0、0.01、0.05、0.1、0.5、1、5、10μmol/L鱼藤酮处理细胞12h,以及1μmol/L鱼藤酮分别处理细胞0、2、6、12、18、24h,Western blot检测各组细胞内LC3-Ⅰ和LC3-Ⅱ含量,荧光显微镜检测GFP-LC3荧光斑点的数量,透射电镜观察细胞超微结构。结果随着鱼藤酮处理浓度升高,LC3-Ⅱ/LC3-Ⅰ比值和GFP-LC3荧光斑点数量均随之增加;随着鱼藤酮处理时间延长,GFP-LC3荧光斑点数量也随之增加,LC3-Ⅱ/LC3-Ⅰ比值先升高后降低,透射电镜显示细胞线粒体受损和细胞自噬水平增加。结论鱼藤酮对PC12细胞自噬的影响具有量效性和时效性。Objective To investigate the effects on autophagy induced by rotenone in PC12 cells. Methods PC12 cells was treated with 0,0. 01,0. 05,0. 1,0. 5,1,5,10 vcmol/L rotenone for 12 hours and with 1 μmol/L rotenone for 0,2,6,12,18,24 hours respectively. Expressions of LC3-I and LC3- II were detected by Western blot and dots of GFP-LC3 were examined by fluorescence microscopy. Cell ultrastructures were observed under transmission electron microscope. Results With increasing concentration of rotenone, the ratios of LC3- II / LC3- I and the numbers of dots increased accordantly. With increasing time of rotenone, the numbers of dots increased accordantly while the ratios of LC3- II / LC3- I rose at first and fell afterwards. The mitochondria were obviously damaged and the level of autophagy was increased in PC12 cells. Conclusion There is a time based and quantitative efficiency in the effects on autophagy induced by rotenone in PC12 cells.

关 键 词：鱼藤酮 自噬 PC12细胞 

分 类 号：R742.5[医药卫生—神经病学与精神病学]