微小RNA-30b对直肠癌细胞系松软蛋白4及程度细胞凋亡基因4表达的影响  

Effect of microRNA- 30b on Sox4 protein and Pdcd4 expression in colorectal cancer cell lines

作　　者：徐芳[1] 殷宏[2] 秦岩[3] 吴凡[4] 王娅南[1] 张娟[5] 

机构地区：[1]河北大学附属医院病理科,河北保定071000 [2]河北大学附属医院检验科,河北保定071000 [3]河北大学附属医院中心实验室,河北保定071000 [4]河北大学附属医院口腔科,河北保定071000 [5]河北医科大学第四医院普外科,石家庄050011

出　　处：《中国临床药理学杂志》2015年第17期1736-1739,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省卫生计生委2014年度医学科学研究重点课题计划基金资助项目(ZL20140038)

摘　　要：目的探讨微小RNA-30b对结肠癌细胞株松软蛋白4(Sox4)及程度细胞凋亡基因4(Pdcd4)表达的影响。方法用10%新生牛血清培养结肠癌HCT116和SW480细胞株,各分为干预组与模型组。p LVTHM/miR-30b慢病毒载体转染,细胞培养基培养6 h。用免疫印迹法与荧光定量PCR法对Sox4及Pdcd4表达进行测定。结果与模型组比较,干预组Sox4整合光密度值明显降低,干预组Pdcd4整合光密度值明显增高(P<0.01);与模型组比较,干预组Sox4蛋白表达较低,干预组Pdcd4表达增高(P<0.01)。结论 MicroRNA-30b能够显著降低直肠癌细胞系Sox4蛋白的表达。Objective To investigate the effect of microRNA- 30 b on the expression of Sox4 and Pdcd4 in colorectal cancer cell lines. Methods Colon cancer HCT116 and SW480 cell lines cultured in 10% newborn bovine serum,were divided into 2 groups trained in cell culture medium for 6 h. The experiment group were transfection by miRNA- 30 b,and cultured in cell culture medium for 6 h. The expression of Pdcd4 and Sox4 were determined by Western blot and polymerase chain reaction（ PCR）. Results Sox4 absorbance of the experiment group was lower,Pdcd4 was higher,compared with the model group（ P 〈 0. 01）. The expression of Sox4 protein of the experiment was lower,Pdcd4 was higher,compared with the model group（ P〈 0. 01）. Conclusion MicroRNA-30 b can significantly reduce the expression of Sox4 protein in colorectal cancer cell lines,Pdcd4 expression increased.

关 键 词：直肠癌细胞系 松软蛋白4 程度细胞凋亡基因4 靶向治疗 微小基因30b 

分 类 号：R363[医药卫生—病理学] R735.35[医药卫生—基础医学]