基于共表达网络研究巨噬细胞在动脉硬化中的作用  

The study of the role of macrophages in atherosclerosis based on the co-expression network

作　　者：赵宏群 张一骅 李琬[1] 何月涵[1] 吕俊杰[1] 黄昊[1] 陈丽娜[1] 

机构地区：[1]哈尔滨医科大学生物信息学院,150081

出　　处：《国际免疫学杂志》2015年第5期409-412,共4页International Journal of Immunology

基　　金：国家自然科学基金（61272388）;黑龙江省自然科学基金（F201237）;国家及黑龙江省大学生创新创业训练基金（201410226010）.

摘　　要：目的探讨巨噬细胞在动脉粥样硬化形成和发展中的作用机理。方法从GEO数据库中下载一组人类冠状动脉粥样硬化（AS）的单核细胞转录组基因表达谱数据。通过Bicor相关方法分别构建基于正常和疾病样本差异基因共表达网络，用ClusterOne挖掘疾病候选功能模块后进行富集分析，对模块之间重叠度及功能富集情况进行分析。结果在疾病和正常网络中分别得到3个功能相关模块，共富集到11个功能类，从正常到疾病状态中模块间基因存在差异，富集到的功能类也存在差异。结论不同模块中基因的突变、凋亡、磷酸化和去磷酸化等在巨噬细胞致AS过程中起着重要作用。Objective To explore the role of macrophages in the formation and development of atherosclerosis. Methods We downloaded a set of human coronary atherosclerosis monocyte gene expression data from the GEO database. We applied the Biweight midcorrelation （Bicor） methods to correlation calculation. The co- expression network was constructed and the corresponding network topology was calculated according to normal samples and disease samples. We also found some disease - related candidate modules through Clus- terOne methods and analyzed the difference of the degree of overlap between the modules and functional enrich- ment analysis of the results. Finally we indicated the pathogenic macrophages in AS. Results Three related modules were enriched to 11 functional categories from control and disease network, respectively. Differences between states from normal to disease in module genes enriched in functional categories were also found. Conclusion Gene mutations, apoptosis, phosphorylation and dephosphorylation etc. in macrophages from different modules played an important role in inducing atherosclerotic process.

关 键 词：动脉粥样硬化 巨噬细胞 共表达网络 Biweight midcorrelation 

分 类 号：R543.5[医药卫生—心血管疾病]