PRL型垂体瘤患者血清miRNA表达谱的检测及初步筛选分析  

Detection of Serum miRNA Expression Profile and the Preliminary Screening and Analysis in the Patients of PRL- Type Hypophysoma

作　　者：张怀璧[1] 徐川[1] 施扬[1] 陈洁[1] 张秋娟[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院神经内科,上海200437

出　　处：《世界中西医结合杂志》2015年第4期543-548,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(81273732/H2708)

摘　　要：目的检测及初步筛选分析PRL型垂体瘤患者血清miRNA表达谱。方法采集20例PRL型垂体瘤患者和20例健康志愿者血清,通过RNA提取技术、miRNA TLDA芯片技术检测血清miRNA、采用软件Data AssistTMSoftware进行数据分析和处理,筛选出差异性表达的miRNA。结果以差异表达量大于2倍,芯片检测的相对定量平均CT值小于40为标准,初步筛选出PRL型垂体瘤患者血清miRNA表达上调的有3个,表达下调的有25个,共计28个。结论 PRL型垂体瘤患者和健康志愿者血清miRNA具有内源表达的差异性,可以为进一步验证特异性血清miRNA的表达,探索药物临床疗效机制研究提供理论和实验依据。Objective To detect and preliminarily screen and analyze serum miRNA expression profile in the patients of PRL - type hypophysoma. Methods The serum was collected from 20 patients of PRL - type hypophysoma and 20 healthy volunteers. RNA extract technology and miRNA TLDA chip technology were used to detect serum miRNA. The Data Assist TM Software was adopted for the analysis and management of data and for screening the differences of miRNA expressions. Results By taking the expression difference （by more than 2 times）and the mean CT value （ 〉 40 ）of the relative quantification detected by chip as the criteria, it was preliminarily screened that 3 miRNA expressions were up - regulated and 25 were down - regulated in the patients of PRL - type hypophysoma, totally 28 expressions were involved. Conclusion The endogenous expressions of serum miRNA were different between the patients of PRL - type hypophysoma and healthy volunteers so that the specificity of serum miRNA expressions can be further verified and the theoretic and experimental evidences be provided for the exploration of clinical efficacy mechanism of medicines.

关 键 词：PRL型垂体瘤 血清miRNA 芯片检测 

分 类 号：R739.41[医药卫生—肿瘤] R736.4[医药卫生—临床医学]