静电纺丝法制备载盐酸多西环素GTR/GBR膜的结构和体外抑菌性能研究  

The investigation of a doxycycline hydrochloride-loaded electrospun GTR/GBR membrane's structure and its antibacterial efficiency

作　　者：贾列妮 徐宏宇 华菲[2] 胡祥刚 谢乔 王伟[1] 贾骏 

机构地区：[1]第四军医大学口腔医院修复科.军事口腔医学国家重点实验室,陕西西安710032 [2]第四军医大学天然药物学教研室,陕西西安710032

出　　处：《临床口腔医学杂志》2015年第9期515-518,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助(81271136)

摘　　要：目的:利用静电纺丝法制备载盐酸多西环素(DOX)纳米纤维膜片,评价其作为局部药物治疗与GTR/GBR技术的结合,在治疗种植体周围炎方面的可行性的相关基础研究。方法:用静电纺丝法制备载DOX的聚己内酯(PCL)纳米纤维膜。扫描电子显微镜观察纤维膜的表面形貌、分析药物对纤维形貌的影响,差示量热扫描法(DSC)分析药物在纤维中的分布状态,琼脂扩散实验检测载药膜片对牙龈卟啉单胞菌(Pg)和伴放线放线杆菌(Aa)的抑菌作用。结果:制备的DOX-PCL纳米纤维表面光滑,直径分布均匀,随着DOX的载药量由15%增加到25%,纤维直径由247.17nm降低到181.34 nm;同时以无定形分子形式分布于纳米纤维中的DOX对Aa和Pg均具有明显的抑菌作用。结论:作为药物治疗与GTR/GBR技术的结合,静电纺丝法制备的载药GTR/GBR膜在种植体周围炎的治疗方面具有良好的应用前景。Objective： To prepare a doxycycline hydrochloride （DOX）-loaded nanofibrous membrane by electrospining method. Evaluate its relative foundational properties for peri-implantitis therapy as the combination of local drug treatment and GTR / GBR surgery. Method： Different groups DOX-loaded PCL nanofibrous membranes were prepared by eletrospun. Then we observed the morphology and the diameter distribution of nanofibers, investigated the manner of DOX in drug-loaded nanofibers and its bioactivity against Aggregatibacter actinomycetemcomitans （Aa） and Porphyromonas gingivalis （Pg）. Result： DOX-loaded PCL nanofibers were uniform distributed and the surface of fibers were smooth. Fibrous average diameters decreased from 247.16 nm to 181.34nm with the drug proportion increasing from 15 % to 25 % w / w. Differential scanning calorimetry （DSC） test showed DOX appeared an amorphous manner in the polymer matrix. And DOX-loaded membranes had an obvious inhibited effect on Aggregatibacter actinomycetemcomitans （A a） and Porphyromonas gingivalis （Pg）, respectively. Conclusion： As the combination of local drug treatment and GTR/GBR surgery, electrospinning DOX-loaded PCL nanofibrous membrane has a well prospect on the field of peri-implantitis therapy.

关 键 词：静电纺丝 盐酸多西环素 载药GTR/GBR膜 种植体周围炎 

分 类 号：R783.1[医药卫生—口腔医学]