CD47和钙网织蛋白在浸润性乳腺导管癌组织中的表达及意义  

The expression and clinical significance of CD47 and calreticulin in infiltrating ductal breast carcinomas

作　　者：陈琪枫[1] 方晓明[1] 胡新根[2] 姜朝晖[1] 张宝燕[2] 姚宁[1] 

机构地区：[1]解放军第一一七医院普通外科,浙江省杭州310013 [2]解放军第一一七医院病理科,浙江省杭州310013

出　　处：《中国基层医药》2015年第17期2574-2577,共4页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省杭州市卫生科技计划项目（2012B056）

摘　　要：目的研究CD47和钙网织蛋白（CRT）在浸润性乳腺导管癌（IDCAs）组织中的表达及与临床病理特征、预后的关系。方法应用免疫组化方法（ICH）对128例IDCAs及其癌旁组织中CD47、CRT的表达进行检测，并分析其与临床病理指标以及预后的关系。结果癌组织中CD47的表达为81／128，显著低于癌旁组织表达的106／128（）（2：12．400，P〈0．05）；CRT的表达为50／128，高于癌旁组织的31／128（x^2=6．520，P〈0．05），且各分子分型问表达差异有统计学意义（x^2=21．510，P〈0．05），基底细胞样型和Her-（2＋）／ER（-）型明显高表达。CD47的表达与肿瘤大小、分化程度、淋巴结转移相关（0=11．400、4．732、5．432，均P〈0．05）；而CRT的表达与肿瘤分化程度、肿瘤远处转移相关（X^2=10．810、6．770，均P〈0．05）。在完成随访的98例患者中，5年生存率为68．37％。单因素生存分析显示CD47、CRT高表达组和低表达组在5年内生存率差异有统计学意义（X^2=5．231、13．069，均P〈0．05），表达越高患者5年内生存率越低。结论CD47、CRT与IDCAs预后差的指标相关，与5年内生存率显著相关，联合检测CD47和CRT对判断乳腺癌的预后有指导价值。Objective To evaluate the expression of CD47 and calreticulin and their relationships with clini- copathologic features, prognosis in infihrating ductal breast carcinomas. Methods The expression of CD47 and calret-iculin was evaluated in 128 cases with infiltrating ductal breast carcinomas by means of immunohistochemistry, and the relationship between CD47 and other clinicopathologic factors as well as prognosis were analyzed. Results The expression level of CD47 in cancer nest was 81/128 ,which was lower than 106/128 in its adjacent normal epithelium （ X^2 = 12. 400 ,P 〈 0.05 ） , but calreticulin expression was 50/128 and was higher in cancer nest （ X2 = 21. 510, P 〈0. 05）. In addition, the expression level of calreticulin had certain difference in different molecular subtype （X^2 = 21. 510 ,P 〈 0.05 ）, and an increased expression in Basal -like and Her - （2 ＋ ）/ER（ - ）subtypes. The expression level of CD47 was associated with tumor sizes, histological tumor grade and lymph node involvement （X^2 = 11. 400, 4. 732,5. 432, all P 〈 0.05 ）, and the expression level of calreticulin was associated with histological tumor grade and distant metastasis （X^2 = 10. 810,6.770, all P 〈 0.05 ）. The 5 -year survival rate of patients performed following-up was 68.37%. Univariate analysis indicated the survival rate in 5 years of high level CD47 ,calreticulin expression was higher than that of low level CD47, calreticulin expression （ X^2 = 5.231,13. 069, all P 〈 0.05 ）. Conclusion The expression of CD47, calreticulin is associated with index of dysprognosis and survival rate in 5 years, so combined CD47 and calreticulin detect may be of prognostic value in patients with breast caner.

关 键 词：CD47 钙网织蛋白 乳腺肿瘤 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]