流式细胞术对2型糖尿病大鼠红细胞凋亡的研究  

Erythrocytes apoptosis of rats with type 2 diabetes mellitus by flow cytometry

作　　者：王磊[1,2] 肖洪彬[1] 牛雯颖[1] 姜广坤[1] 冯月楠 卞敬琦[1] 乔玉良[1] 

机构地区：[1]黑龙江中医药大学教学实验中心,黑龙江哈尔滨150040 [2]哈尔滨技师学院,黑龙江哈尔滨150030

出　　处：《中国医学物理学杂志》2015年第5期660-663,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(81173186;81473555)

摘　　要：目的:采用Annexin V-FITC/PI双染色、激光流式细胞术(Annexin V-FITC-FITC/PI-FCM)对2型糖尿病大鼠红细胞、老龄大鼠红细胞、正常大鼠红细胞的凋亡情况进行研究,同时分析上述模型的血液流变情况,对比病理情况和生理情况对红细胞凋亡和血液流变学的影响。方法:采用Annexin V-FITC/PI双染色、激光流式细胞术对3种大鼠模型红细胞凋亡情况进行测试,用Z血液流变分析仪对3种大鼠模型的血液粘度进行分析。结果:糖尿病大鼠红细胞存在凋亡,与此同时糖尿病大鼠血液粘度明显增大。结论:糖尿病大鼠红细胞存在凋亡,老龄大鼠和正常大鼠红细胞不存在凋亡,且凋亡与年龄无关,红细胞凋亡与血液粘度之间存在关联性。Objective To analyze the blood rheological of three rat models built in this paper, and compare the effect of physiological and pathological conditions on the erythrocytes apoptosis and blood rheological by researching the erythrocytes apoptosis of rats with type 2 diabetes mellitus, aging rats and normal rats based on Annexin V-FITC/PI double-staining and Annexin V- FITC- FITC/PI- FCM. Methods Annexin V- FITC/PI doublestaining and Annexin V- FITC-FITC/PI- FCM were applied to study on the erythrocytes apoptosis of three rat models, rats with rats with type 2 diabetes mellitus, aging rats and normal rats. The blood viscosities of these three rat models were analyzed by Z hemorheology analyzer. Results Apoptosis appeared in the erythrocytes of diabetic rats and the blood viscosity of diabetic rats increased significantly. Conclusion Apoptosis appears in the erythrocytes of diabetic rats, not in the erythrocytes of aging rats and normal rats. And the apoptosis is not associated with age. However, there are correlations between erythrocytes apoptosis and blood viscosity.

关 键 词：流式细胞术 2型糖尿病 红细胞 凋亡 

分 类 号：R363[医药卫生—病理学] R587.1[医药卫生—基础医学]