5-ALA介导的光动力疗法对人舌鳞癌Tca8113细胞增殖、凋亡及细胞周期的影响  

Effects of 5-ALA mediated photodynamic therapy on proliferation,apoptosis and cell cycle of human tongue squamous carcinoma Tca8113 cells

作　　者：王桃[1] 刘伟伟 谢冰[3] 张瑞[3] 张朋[1] 

机构地区：[1]郑州大学口腔医学院修复科,郑州450052 [2]郑州市口腔医院综合科,郑州450099 [3]郑州大学附属郑州中心医院口腔科,郑州450007

出　　处：《郑州大学学报（医学版）》2015年第4期500-503,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：郑州市重点科技攻关基金资助项目121PPTGG504-8

摘　　要：目的:探讨5-氨基酮戊酸介导的光动力疗法(5-ALA-PDT)对体外培养的人舌鳞癌Tca8113细胞增殖、凋亡及细胞周期的影响。方法:体外培养Tca8113细胞,以5-ALA为光敏剂,半导体激光治疗仪给予光照,设立对照组、低浓度5-ALA-PDT组(0.05 mmol/L 5-ALA+PDT治疗)、高浓度5-ALA-PDT组(5.00 mmol/L 5-ALA+PDT治疗),镜下观察细胞形态的改变;5-ALA浓度分别设定为0.01、0.05、0.10、0.50、1.00、5.00、10.00 mmol/L,各浓度组依次孵育1、2、3、4、6、8 h,然后给予光照刺激,同时设立对照组,MTT法检测细胞增殖的变化;设置O组(正常对照组)、A组(0.05 mmol/L 5-ALA)、B组(0.50 mmol/L 5-ALA)、C组(5.00 mmol/L 5-ALA),给予光照刺激,流式细胞术检测细胞凋亡情况;设置D组(5-ALA-PDT干预组),E组(对照组),流式细胞术检测细胞周期的改变。结果:镜下可见5-ALA-PDT作用后细胞形态改变,细胞皱缩变圆。MTT法检测结果显示5-ALA-PDT可抑制Tca8113细胞增殖(F5-ALA=2 859.796,F时间=237.820,F交互=16.687,P均<0.001)。B、C组与O组比较,早期凋亡率、晚期凋亡率、坏死率增加(P均<0.05)。5-ALA-PDT作用后G0/G1期细胞多于对照组,G2/M期、S期细胞少于对照组(t=57.275、7.164、45.157,P均<0.001)。结论:5-ALA-PDT能抑制Tca8113细胞增殖,诱导细胞凋亡,并将细胞阻滞在G0/G1期。Aim： To study the proliferation, apoptosis, and cell cycle of human tongue squamous cell carcinoma Tca8113 cells induced by 5-aminolevulinic acid（5-ALA） mediated photodynamic therapy（ PDT）. Methods： Tca8113 cells, cultured in vitro, were induced by 5-ALA and semiconductor laser. These cells were divided into control group, low concentration group, and high concentration group. Then the microscope was used to observe the form changes of cells af-fected by 5-ALA-PDT. Then, cells were divided into different concentrations （0. 01, 0. 05, 0. 10, 0. 50, 1. 00, 5. 00, 10. 00 mmol/ L 5-ALA） and they were incubated for different time（1, 2, 3, 4, 6, 8 h）, MTT method was used to detect the proliferation of Tca8113 cells. Tca8113 cells were divided into 4 groups： group A（0. 05 mmol/ L 5-ALA）, group B （0. 50 mmol/ L 5-ALA）, group C（5. 00 mmol/ L 5-ALA）, and group O（control group）,and the flow cytometry was used to detect apoptosis. Tca8113 cells were allocated into 5-ALA-PDT group and control group, and the cell cycle was detected＆amp;nbsp;by flow cytometry. Results： The cell form changed and shrank under microscope after using of 5-ALA-PDT. The results of MTT method showed that 5-ALA-PDT could inhibit proliferation of Tca8113 cells（F5-ALA = 2 859. 796,Ftime = 237. 820, F interaction = 16. 687,P 〈 0. 001）. Comparing group B, C with group O, the percentage of early apoptosis, late apoptosis, and necrosis was higher（P 〈 0. 05）. The percentage of G0 / G1 in 5-ALA-PDT group was higher than that of the control group,and the percentage of G2 / M and S was lower（t = 57. 275, 7. 164, 45. 157,P 〈 0. 001）. Conclusion： 5-ALA-PDT could restrain the proliferation of Tca8113 cells, induce apoptosis, and block proliferation at G0 / G1 phase of cell cycle.

关 键 词：5-氨基酮戊酸 光动力疗法 TCA8113 细胞 增殖 凋亡 细胞周期 

分 类 号：R739.8[医药卫生—肿瘤]