人参皂苷Rd对高糖致人牙周膜干细胞成骨分化抑制作用的影响  

Effects of ginsenoside Rd on osteogenic differentiation of high glucose-induced human periodontal ligament stem cells

作　　者：王慧丽[1] 谢冰[1] 李欣[1] 蔺燕萍[1] 张朋[2] 

机构地区：[1]郑州大学附属郑州中心医院口腔科,郑州450007 [2]郑州大学口腔医学院修复科,郑州450052

出　　处：《郑州大学学报（医学版）》2015年第4期542-546,共5页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨高糖诱导条件对人牙周膜干细胞(PDLSCs)增殖及分化的影响,并探讨人参皂苷Rd(GSRd)对高糖诱导PDLSCs成骨分化的影响。方法:PDLSCs经高糖(30 mmol/L D-葡萄糖)或对照(5 mmol/L D-葡萄糖)处理后,通过测定PDLSCs的碱性磷酸酶(ALP)活性、骨钙蛋白(OCN)和ALP的mRNA表达及骨桥蛋白(OPN)的蛋白表达,以评估PDLSCs成骨分化水平。同时通过检测GSRd对PDLSCs经高糖处理后超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的mRNA及蛋白表达水平,以评估GSRd对高糖诱导PDLSCs成骨分化中氧化应激的影响。结果:高糖处理可减低PDLSCs中ALP的活性(P<0.05),而10和40μmol/L的GSRd可提高正常和高糖培养条件下PDLSCs中ALP的活性(P<0.05);并且,GSRd可上调ALP、OCN mRNA表达及OPN蛋白表达(F=102.893、56.061、60.296,P<0.05),上调CAT、SOD的mRNA及蛋白表达水平(F=48.393、67.134、29.837、7.539,P<0.05)。结论:GSRd对体外培养的PDLSCs的成骨分化有一定的促进作用;在高糖条件,GSRd可以减轻氧化应激对PDLSCs成骨的抑制作用,促进PDLSCs的成骨作用。Aim： To explore the effects of ginsenoside Rd（ GSRd） on osteogenic differentiation of high glucose-in-duced human periodontal ligament stem cells（PDLSCs）. Methods： PDLSCs were pretreated with 30 mmol/ L D-glucose and 5 mmol/ L D-glucose, then the osteogenic differentiation indexes of PDLSCs such as the ALP activity, mRNA expres-sions of ALP, OCN, and protein expression of OPN were detected. To evaluate the effects of GSRd on oxidative stress of high glucose-induced PDLSCs, the protein and mRNA expressions of SOD and CAT were detected. Results： High glucose treating decreased the ALP activity of PDLSCs（P 〈 0. 05）, but 10 and 40 μmol/ L GSRd could improve the ALP activity of PDLSCs significantly（P 〈 0. 05）. Compared with the control group, GSRd upregulated the mRNA expressions of ALP, OCN, and protein expression of OPN（F = 102. 893, 56. 061, 60. 296, P 〈 0. 05）. GSRd also upregulated the mRNA and protein expressions of CAT and SOD（F = 48. 393, 67. 134, 29. 837, 7. 539,P 〈 0. 05）. Conclusion： GSRd could improve the osteogenic differentiation of PDLSCs and reduce the oxidative stress of PDLSCs with high glucose treatment, and then improve the osteogenic differentiation of PDLSCs.

关 键 词：人参皂苷RD 牙周膜干细胞 成骨分化 氧化应激 

分 类 号：R781.4[医药卫生—口腔医学]