脑梗死大鼠缺血脑组织中Slit2蛋白的表达及与血管新生的关系  

Expression of Slit2 protein in infarction brain tissue and its correlation with angiogenesis in rats with cerebral infarction

作　　者：刘海燕[1] 娄季宇[1] 白宏英[1] 曾志磊[1] 闫莹莹[1] 乐婷[1] 

机构地区：[1]郑州大学第二附属医院神经内科,郑州450014

出　　处：《郑州大学学报（医学版）》2015年第5期655-658,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨Slit2/Robo1信号通路在脑缺血后血管新生中的作用。方法:雄性SD大鼠48只,随机分为假手术组和模型1、3、7天组,每组12只。模型1、3、7天组采用线栓法制备大鼠永久性大脑中动脉缺血模型并分别于造模后第1、3、7天进行指标检测。应用Western blot方法检测缺血脑组织中Slit2蛋白的表达。应用RT-PCR方法检测缺血脑组织中Robo1、血管内皮生长因子(VEGF)mRNA的表达。利用CD105标记新生血管内皮细胞,计数大鼠脑组织中微血管密度(MVD)。结果:4组大鼠缺血脑组织中Slit2蛋白、Robo1和VEGF mRNA的表达及MVD差异有统计学意义(F=394.528、1 058.653、898.938和142.753,P<0.001)。模型1、3、7天组Slit2蛋白、VEGF mRNA表达水平及MVD均明显高于假手术组(P<0.05),并且逐渐升高(P<0.05);Robo1 mRNA的表达水平在3组间逐渐升高(P<0.05),但均低于假手术组(P<0.05)。模型组大鼠Slit2蛋白表达水平与VEGF mRNA表达水平、MVD呈正相关(r=0.974、0.926,P<0.001)。结论:Slit/Robo信号通路可能参与了大鼠脑缺血后的血管新生过程。Aim：To explore the relationship between Slit 2/Robo1 signaling pathway and angiogenesis after ischemia . Methods：Forty-eight male SD rats were randomly allocated into sham operation group and D 1,D3,D7 model groups（n=12）.The D1,D3,D7 model groups were to establish cerebral ischemia model by the suture method .Western blot was used to analyze the Slit2 expression in infarction brain tissue and RT-PCR was used to detect the expressions of Robo 1 and VEGF mRNA.Microvessel density （ MVD） was calculated through marking the vessel endothelial cells with CD 105.Results：There were significant differences in the Slit 2 protein, Robo1 and VEGF mRNA expressions ,and MVD among the 4 groups （F=394.528,1 058.653,898.938 and 142.753,P〈0.001）.The expressions of Slit2 protein, VEGF mRNA and MVD in＆amp;nbsp;D1,D3,D7 model groups were higher than that of the sham group , and increased by turn（P〈0.05）; the Robo1 mRNA expression were increased by turn（P〈0.05）,but still lower than that of the sham operation operation group .In the 3 model groups, the Slit2 protein expression was positively correlated with VEGF mRNA and MVD （r=0.974,0.926,P〈0.001）. Conclusion：Slit2/Robo1 signaling pathway may be involved in the process of angiogenesis after focal cerebral ischemia .

关 键 词：SLIT2 ROBO1 血管新生 血管内皮生长因子 脑梗死 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]