聚合Stv-b^i和Wx-mq基因改良武育粳3号条纹叶枯病抗性和食味品质  

Improving Resistance to Rice Stripe Disease and Eating Quality of Wuyujing 3 by Pyramiding Stv-b^i and Wx-mq

作　　者：陈涛[1] 吴昊[1] 张亚东[1] 朱镇[1] 赵庆勇[1] 周丽慧[1] 姚姝[1] 赵凌[1] 于新[1] 赵春芳[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,南京210014

出　　处：《中国水稻科学》2015年第5期467-474,共8页Chinese Journal of Rice Science

基　　金：现代农业产业技术体系建设专项基金资助项目(CARS-01-47);江苏省农业科技自主创新资金项目(CX[12]1003);江苏省科技支撑计划资助项目(BE2012303)

摘　　要：以日本优质粳稻关东194为条纹叶枯病抗性基因Stv-bi和低直链淀粉含量基因Wx-mq的供体,采用回交育种的策略,改良武育粳3号的抗性和食味品质。在连续回交和自交的过程中,利用与Stv-bi和Wx-mq基因相关的分子标记进行跟踪检测,并结合条纹叶枯病的田间抗性鉴定以及农艺性状的系统选择,在BC3F4世代中筛选到10个具有Stv-bi Stv-bi WxmqWx-mq纯合基因型的改良系。性状分析表明,这些品系保留了受体亲本武育粳3号农艺性状,条纹叶枯病抗性得到明显改良,同时,食味、外观品质也大幅提升。综合分析,最终筛选出K01和K04两个优异品系。The japonica rice variety Kantou 194 was used as the donor of the resistance gene Stv-bi and the low-amylose content gene Wx-mq to improve the resistance and eating quality of Wuyujing 3by backcross.In continuous backcross and selfcross,molecular markers linked with Stv-b^i and Wx-mq were utilized for genotypic detection in combination with resistance identification to rice stripe disease and agronomic trait selection.Finally,10 lines with homozygous genotype Stv-b^i Stv-b^i Wx-mqWx-mq were obtained from BC3 F4 generation.The results of comparative analysis indicated that with similar agronomic traits to the receptor parent Wuyujing 3,those lines had substantial improments in resistance,appearance and eating quality.By comprehensive evaluation,two excellent lines K01 and K04 were selected.

关 键 词：水稻 条纹叶枯病 食味品质 Stv-b^i基因 Wx-mq基因 聚合育种 

分 类 号：S511.22[农业科学—作物学]