莪术醇对胃癌SGC7901细胞凋亡及AIF、Endo G表达的影响  

Effect of Curcumol on Apoptosis and Expressions of AIF,Endo G in Gastric Cancer SGC7901 Cells

作　　者：张弘[1] 张连荣 姜海军[1] 高建国[1] 杜建青[1] 杨植[1] 

机构地区：[1]承德医学院附属医院,河北承德067000 [2]承德市第六医院,河北承德067400

出　　处：《肿瘤学杂志》2015年第9期703-707,共5页Journal of Chinese Oncology

基　　金：河北省医学科学研究重点课题计划项目(20120166)

摘　　要：[目的]观察莪术醇对胃癌细胞株SGC7901细胞凋亡及凋亡诱导因子(AIF)和核酸内切酶G(Endo G)表达的影响,探讨其作用机制。[方法]对体外培养的SGC7901细胞,分别加入不同浓度的莪术醇处理,采用四甲基偶氮唑蓝(MTT)法测定增殖抑制率;流式细胞仪检测细胞凋亡率;Western blot方法检测AIF、Endo G蛋白的表达水平。利用Caspase-3抑制剂ZVAD-FMK进一步确定AIF和Endo G在莪术醇抑制胃癌SGC7901细胞凋亡的机制。[结果 ]莪术醇能显著抑制SGC7901细胞增殖;与对照组比较,莪术醇组细胞凋亡率明显升高(P<0.01),AIF、Endo G蛋白表达明显升高(P<0.05,P<0.01);与对照组比较,同时给予莪术醇和ZVAD-FMK组细胞凋亡率明显升高(P<0.01),AIF、Endo G蛋白表达明显升高(P<0.01);但与莪术醇组比较无显著差异。[结论]莪术醇可上调AIF、Endo G表达,通过非Caspase依赖通路诱导胃癌SGC7901细胞凋亡。[Purpose] To investigate the effect of Curcumol on apoptosis and expressions of apoptosis inducing factor（AIF） and endonuclease G（Endo G） proteins in human gastric cancer SGC7901 cells and their molecular mechanisms. [Methods] SGC7901 cells were cultured and treated with different Curcumol concentrations. The growth inhibition was tested by thiazolyl blue terazolium bromide（MTT） assay. Flow cytometry（FCM） was used to measure the cell apoptotic rate.Protein expressions of AIF and Endo G were measured by Western blot. Z-VAD-FMK（Caspase-3inhibitor） was applied to evaluate the mechanism of AIF,Endo G in the process of Curcumol inhibiting apoptosis of gastric cancer SGC7901 cells.[Results] Curcumol inhibited the cell proliferation of SGC7901 cells. Compared with the control group,the apoptotic rate（P 0.01）,AIF and Endo G protein expressions（P〈0.05,P〈0.01） increased in Curcumol group. Compared with the control group,the apoptotic rate（P〈0.01）,AIF and Endo G protein expressions（P〈0.01） increased in Curcumol combined with Z-VAD-FMK group. But there was not significant difference between Curcumol group and Curcumol combined with Z-VAD-FMK group. [Conclusion] Curcumol might upregulate the expressions of AIF and Endo G,and induce human gastric cancer SGC7901 cell apoptosis through Caspase-independent pathway.

关 键 词：莪术醇 胃肿瘤 细胞凋亡 半胱氨酸蛋白酶 凋亡诱导因子 核酸内切酶G 

分 类 号：R735.2[医药卫生—肿瘤]