马铃薯甜菜碱醛基因PoBADH的克隆与进化分析  

Cloning and Evolutional Analysis of PoBADH in Potato( Solanum tuberosum)

作　　者：陈晓军[1] 樊云芳 王敬东[1] 郭生虎[1] 宋玉霞[1] 

机构地区：[1]宁夏农业生物技术重点实验室/宁夏农林科学院农业生物技术研究中心,宁夏银川750002 [2]国家枸杞工程技术研究中心,宁夏银川750002

出　　处：《核农学报》2015年第7期1271-1277,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家科技支撑计划课题(2009BADC5B04)

摘　　要：为了揭示马铃薯适应盐生环境和耐盐的分子机制,本研究以青薯168为试验材料,利用RACE同源克隆技术,设计简并引物,克隆得到1个新的甜菜碱醛脱氢酶基因(Po BADH)。它全长c DNA编码区为1 518 bp,编码区两侧翼分别具有5'UTR(76bp)和3'UTR(196bp),推测该开放阅读框编码505个氨基酸的BADH蛋白,其分子量为55 903.1,理论等电点为5.03。在推导的氨基酸序列中,含有醛脱氢酶所具有的高度保守的十肽(VTLELGGKSP)以及与酶功能有关的半胱氨酸残基(C)。在N端含有不典型的信号肽QLFIDGE,表明该酶可能定位于叶绿体中;在C端具有一个SKL型信号肽,可能是定位于过氧化物酶体中的信号肽。进化分析表明,它与番茄甜菜碱醛脱氢酶亲缘关系最近,与其它藜科植物亲缘关系较远。该基因的获得有助于理解马铃薯耐盐机理,为培育新的耐盐马铃薯品种提供理论参考。In order to understand the adapted ability under the salt environment and mechanism of potato salt - tolerance, a new gene of Betaine Aldehyde Dehydrogenase （BADH） , named PoBADH, was obtained by RACE technology with degenerate primer from QinShu168 （Solanum tuberosum）. The full cDNA of PoBADH is consisted of 1 518 bp ORF which encodes 505 putative amino acids , 5＇UTR （76bp） and 3＇UTR （196bp） flanking sequences . A high conserved decapeptide sequence ＇VTLELGGKSP＇ and Cys which is related to the activity of enzyme, are founded in putative amino acid sequence. An atypical signal peptide QLFIDGE in N end indicated that this enzyme maybe locate in chloroplast. Another signal peptide SKL in C end implied that this protein may also play in peroxisome. PoBADH shown closer related to Solanum lycopersicum than other plants in genetic relationship with evolutional analysis. The obtaining of PoBADH2 will help to understand the mechanism of salt tolerance and provide base for developing new salt - tolerance variety in potato （Solanum tuberosum）.

关 键 词：马铃薯 甜菜碱醛脱氢酶 基因克隆 进化分析 

分 类 号：S532[农业科学—作物学]