气质联用-模式识别对甲嘎松汤质量控制的方法研究  

Pattern Recognition Analysis for Quality Control of Jia Ga Song Tang by GC-MS

作　　者：谭庆龙[1,2] 熊天琴[1] 谢丽霞[1] 廖嘉仪[1] 杨滔[1,2] 林曦[1] 张翠仙[1,2] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广中尖峰天然产物共建实验室,广东广州510006

出　　处：《中药材》2015年第5期1056-1059,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81202997);广东省科技计划项目(2011B031700075);广东省自然科学基金(S2011010003925);广州中医药大学科学研究基金(2004D25)

摘　　要：目的:建立藏方甲嘎松汤的质量评价方法。方法:通过水蒸气蒸馏法提取甲嘎松汤挥发油样品,采用气相色谱-质谱(GC-MS)联用技术对挥发油进行分析,并转化成数据矩阵,采用主成分分析以及系统聚类分析进行化学模式识别研究。结果:经聚类分析可将16批甲嘎松汤挥发油样品分为2大类,经主成分分析可将甲嘎松汤综合为3个主成分,可分为2大类;2种模式识别方法对不同甲嘎松汤挥发油样品能准确识别,且其识别结果一致。结论:气质联用-模式识别是一种科学、准确、有效的甲嘎松汤质量控制和评价方法。Objective：To establish a scientific method for identification and evaluation of the Tibetan prescription Jia Ga Song Tang. Methods ： Volatile oil was extracted by water steam distillation and analyzed by GC-MS. Principal component analysis （ PCA ） and hierarchical cluster analysis （HCA）were applied to the samples for chemical fingerprint pattern recognition research. Resuhs ： 16 samples according to hierarchical cluster analysis （HCA）and principal component analysis （PCA）were divided into two classes, and results from two recognition analysis methods had good consistency. Conclusion ： GC-MS-pattern recognition method was a kind of scientific, accurate and effective method for the quality evaluation of Jia Ga Song Tang.

关 键 词：甲嘎松汤 气相色谱-质谱联用 模式识别 系统聚类分析 主成分分析 

分 类 号：R286.2[医药卫生—中药学]