STMN1联合P16、Ki67蛋白检测对宫颈高级别上皮内瘤变的诊断价值  

Value of STMN 1,preiten P16 and Ki-67 in the diagnosis of high-grade cervical intraepithelial neoplasia

作　　者：李燚[1] 岳瑞芹 王红英[2] 

机构地区：[1]山东省聊城市第二人民医院,252601 [2]聊城市人民医院

出　　处：《中国妇产科临床杂志》2015年第5期399-401,共3页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：山东省聊城市卫生局立项课题(聊卫医函【2014】2号-149)

摘　　要：目的本研究通过测定不同宫颈上皮组织中STMN 1、P16及Ki67蛋白的表达情况,探讨联合上述免疫组织化学检查对高级别上皮内瘤变的诊断价值。方法选取宫颈活检或锥切的宫颈标本共150例(其中宫颈炎组织、LSIL组、宫颈癌组织各30例,HSIL 60例),分别检测STMN 1、P16及Ki67蛋白的阳性表达并行统计学分析。结果 STMN 1蛋白主要在细胞浆内阳性染色,为弥漫性或小片状分布,呈浅黄至棕黄色渐变,STMN 1阳性率分别为宫颈炎组23.33%(7/30)、LSIL 60.00%(18/30)、HSIL 93.33%(56/60)、宫颈癌组100%(30/30),呈正相关趋势,STMN 1在LSIL与在HSIL的表达有显著差异(Diff=23.68,Q=5.26,Q>3.633)。结论联合STMN 1、P16及Ki67进行免疫组织化学检查后,较单一免疫组化检查可以更有效的筛选CIN级别,进而提升病理诊断准确率,指导临床治疗。Objective To investigate the expression and significance of STMN 1, P16 and Ki-67 in CIN and cervix carcinoma and to detect whether STMN 1 could be used as a diagnostic marker in cervical SILs. Methods A total of 150 cervical samples, including cervicitis （n = 30）, uterine cervix cancer （n = 30） and squamous lesions, were evaluated for STMN 1, P16, and Ki67 expression by immunohistochemical. CIN were independently scored and classified as low-grade SIL （LSIL/CIN1, n = 30） and HSIL （including CIN2 and CIN3, n = 60） on the basis of a majority diagnosis. Results STMN 1 was positive in 23.33% （7/30） cervicitis, 60% （18/30） LSIL, 93.33% （56/60） HSIL and all uterine cervix cancer （30/30） cases. STMN 1 expression was significantly different between LSIL and HSIL group （Diff= 23.68, Q = 5.26, Q 〉 3.633）. Conclusion Combine STMN I, PI6 and Ki-67 may be a useful method for early diagnosis of cervix carcinoma.

关 键 词：STMN 1 P16 KI67 宫颈上皮内瘤变 免疫组化 

分 类 号：R737.33[医药卫生—肿瘤]