大蒜新素减少Rac1/Cdc42、MMP2表达抑制Hela细胞转移能力的研究  

Allicin Inhibits Metastasis of Hela Cells By Down-regulating Expression of Rac1/Cdc42 and MMP2

作　　者：宋旭日[1] 胡静[1] 张松波[1] 郭中奎 滕淑静[1] 

机构地区：[1]岳阳职业技术学院,湖南岳阳414000 [2]漯河医学高等专科学校,河南漯河462000

出　　处：《动物医学进展》2015年第8期64-67,共4页Progress In Veterinary Medicine

基　　金：湖南省教育厅科研项目(10C0334;13C980)

摘　　要：为探讨大蒜新素对Hela细胞转移能力的抑制作用,采用100μmol/L的大蒜新素处理Hela细胞24h(以等量的PBS为对照),Western blot检测Rac1和Cdc42蛋白表达,半定量RT-PCR法检测MMP2mRNA的表达。结果显示,大蒜新素处理组Hela细胞Rac1和Cdc42蛋白表达明显降低(P<0.05),MMP2mRNA表达明显下降(P<0.01)。说明大蒜新素抑制Hela细胞的转移能力可能与减少Rac1/Cdc42、MMP2的表达密切相关。To investigate the possible mechanism that allicin inhibits Hela cell metastasis,Hela cells were treated with allicin(100 μmol/L).The expressions of Rac1 and Cdc42 proteins were detected by Western blot.The expression of MMP2 mRNA was detected by semi-quantitative RT-PCR method.The expres-sions of Rac1 and Cdc42 in Hela cells treated with allicin were remarkably down-regulated(P <0.05),and the expression of MMP2 mRNA also decreased significantly (P <0.01).The inhibition of Hela cell metas-tasis by allicin may be closely related to changes in expressions of Rac1/Cdc42,MMP2.

关 键 词：大蒜新素 HELA细胞 转移 

分 类 号：Q786[生物学—分子生物学] S633[农业科学—蔬菜学]