多环芳烃诱发小鼠实体瘤及其对白介素-2及白介素-6的影响  

Solid Tumors Induced by Polycyclic Aromatic Hydrocarbons in Mice and Relevant Effects on Interleukin-2 and Interleukin-6

作　　者：王秋兰[1] 万学中[1] 

机构地区：[1]甘肃中医药大学临床医学院,甘肃730000

出　　处：《环境与职业医学》2015年第9期880-883,共4页Journal of Environmental and Occupational Medicine

基　　金：甘肃省自然科学基金项目(编号:145RJZA235);甘肃省高等学校基本科研业务费项目(编号:2012-5)

摘　　要：[目的]探索多环芳烃中单一标准品蒽、苯并[a]芘(Ba P)、苯并[g,h,i]苝诱发小鼠实体瘤的情况;通过白介素-2(IL-2)、白介素-6(IL-6)的测定,探索其对机体免疫功能的影响。[方法]无特定病原体(SPF)级昆明种小鼠120只,雌雄各半,体重(20±2)g。取20只预实验确定染毒剂量,其余100只随机分为5组,每组20只,分别为二甲基亚砜对照组、蒽50 mg/kg组、Ba P 10mg/kg组、Ba P 20mg/kg组,苯并[g,h,i]苝5 mg/kg组,腹腔注射,每日一次,连续10次。3个月后采血,酶联免疫吸附试验(ELISA)测定血清IL-2及IL-6。取肝、肾、胃、肺,进行病理组织学检查。[结果]苯并[g,h,i]苝组5 mg/kg组、Ba P 10 mg/kg组、Ba P 20 mg/kg、蒽50 mg/kg组小鼠肝癌、胃癌、肾癌发生率分别依次为21.1%(4/19)、26.3%(5/19)、35.3%(6/17)、27.8%(5/18),21.1%(4/19)、0.0%(0/19)、41.2%(7/17)、0.0%(0/18),0.0%(0/19)、0.0%(0/19)、11.8%(2/17)、0.0%(0/18);肝癌、胃癌、肾癌癌前病变率依次为68.4%(13/19)、73.7%(14/19)、64.7%(11/17)、55.6%(10/18),78.9%(15/19)、68.4%(13/19)、29.4%(5/17)、27.8%(5/18),42.1%(8/19)、47.4%(9/19)、58.8%(10/17)、33.3%(6/18)。各实验组小鼠癌前病变率均高于对照组(P<0.05);Ba P 20 mg/kg组胃癌发生率及癌前病变率高于10 mg/kg组(P<0.05);实验组肺脏未见明显损害。各实验组小鼠血清IL-2、IL-6水平均低于对照组(P<0.01)。[结论]蒽、Ba P和苯并[g,h,i]苝腹腔注射后对肝脏、胃和肾脏均有明显的损害,肺脏未出现病变,提示多环芳烃的靶器官不同。实验各组IL-2、IL-6浓度均明显低于对照组,提示多环芳烃对机体的免疫功能有明显的抑制作用。推测苯并[g,h,i]苝致癌性最强,其次为Ba P,蒽致癌性最低。[ Objective ] To observe solid tumors in mice induced by single standards of polycyclic aromatic hydrocarbons including anthracene, benzo[a]pyrene, benzo[g, h, i]perylene, and to explore relevant effects on immune function by measuring serum interleukin-2 （IL-2） and interleukin-6 （IL-6）. [ Methods ] The study used a total of 120 specific pathogen free （SPF） Kunming mice, half male and half female, with body weights at （20 ＋ 2） g. Twenty mice were used for pilot experiment to determine dosage. Then the remaining 100 mice were randomly divided into dimethyl sulfoxide control group, anthracene 50mg/kg group, benzo[a]pyrene 10 mg/kg group, benzo[a]pyrene 20 mg/kg group, and benzo[g, h, i]perylene 5 mg/kg group, with 20 mice in each group. The five groups were injected with assigned chemicals intraperitoneally daily for 10 days. Blood sample was collected after three months and serum IL-2 ＆ IL-6 were detected by enzyme linked immunosorbent assay （ELISA）. Liver, kidney, stomach, and lung of mice were subjected to histopathological examination. [ Results ] Of the mice in the benzo[g, h, i]perylene 5 mg/kg group, benzo[a]pyrene 10mg/kg group, benzo[a]pyrene 20mg/kg group, and anthracene 50mg/kg group, the cancer incidences in liver were 21.1% （4/19）, 26.3% （5/19）, 35.3% （6/17）, and 27.8% （5/18）, respectively; the incidences in stomach were 21.1% （4/19）, 0.0% （0/19）, 41.2% （7/17）, and 0.0% （0/18）, respectively; the incidences in kidney were 0.0% （0/19）, 0.0% （0/19）, 11.8% （2/17）, and 0.0% （0/18）, respectively. The occurrences were 68.4% （13/19）, 73.7% （14/19）, 64.7% （11/17）, and 55.6% （10/18） for liver precancerous lesions, 78.9% （15/19）, 68.4% （13/19）, 29.4% （5/17）, and 27.8% （5/18） for stomach precancerous lesions, and 42.1% （8/19）, 47.4% （9/19）, 58.8% （10/17）, and 33.3% （6/18） for kidney precancerous lesions, respectively. The occurrences of precancerous lesions were all significantly higher than thos

关 键 词：多环芳烃 蒽 苯并[A]芘 苯并[g h i]苝 小鼠 实体瘤 白介素-2 白介素-6 

分 类 号：R-332[医药卫生] R114