高通量测序分析口服抗生素大鼠肠道菌群组成变化  

The high-throughput sequencing analysis of intestinal microbiota in rats administered antibiotics orally

作　　者：陈凤[1] 刘爽[2] 王龙娟[1] 范晓磊[1] 孙文长[1] 

机构地区：[1]微生物学教研室,大连医科大学,辽宁大连116044 [2]劳动卫生与环境卫生学教研室,大连医科大学,辽宁大连116044

出　　处：《中国微生态学杂志》2015年第9期1019-1021,共3页Chinese Journal of Microecology

基　　金：辽宁省自然科学基金(2014023049)

摘　　要：目的探讨口服抗生素大鼠肠道菌群变化。方法在雄性SD大鼠的饮用水中添加肠道不吸收的抗生素(新霉素、杆菌肽和游霉素),饮用1周后,收集粪便并提取细菌基因组DNA,PCR扩增r DNA的V4区特定序列,用Illumina Mi Seq平台测序,进行生物信息学分析大鼠粪便中肠道菌群的组成。结果与对照组相比,抗生素组样品的菌群多样性显著下降(P<0.05)。克雷伯菌属、理研菌科等菌群百分比显著增高,乳杆菌属、拟杆菌目(S24-7)等菌群百分比显著下降。结论饮用抗生素的大鼠肠道菌群有显著变化。Objective To explore the changes of intestinal microbiota in rats administered antibiotics orally. Methods Rats were fed with a mixture of nonabsorbable antimicrobials（ ATM,containing neomycin,bacitracin,and natamycin） via drinking water for 7 days. The genomic DNA was extracted from fecal samples. The specific V4 regions of rDNA were amplified by PCR and sequenced by Illumina Mi Seq platform. The changes of intestinal microbiota were analyzed using bioinformatics methods. Results Compared with the control group,the microbiota diversity of the ATM group decreased significantly（ P 〈0. 05）. The percentages of Klebsiella and Rikenellaceae increased significantly while the percentages of Lactobacillus and Bacteriodales（ S24-7） decreased significantly. Conclusion The intestinal microbiota of rats changed significantly after oral antibiotic treatment.

关 键 词：肠道菌群 抗生素 高通量组测序 

分 类 号：R575.2[医药卫生—消化系统]