嗜水气单胞菌毒力因子检测及不同毒力基因型菌株对剑尾鱼的致病性研究  

Detection of virulence genes in Aeromonas hydrophila and the pathogenicity of different genotype isolates to swordtail

作　　者：任燕[1] 孙承文[1] 石存斌[1] 潘厚军[1] 陶家发[1] 吴淑勤[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所/农业部渔药创制重点实验室/广东省水产动物免疫技术重点实验室,广东广州510380

出　　处：《广东农业科学》2015年第17期113-117,共5页Guangdong Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(2008 03013);国家科技支撑计划项目(2012BAD25B02);国家现代农业产业技术体系建设专项(CARS-46)

摘　　要：选取嗜水气单胞菌(Aeromonas hydrophila,Ah)的4种主要胞外毒力因子(气溶素、溶血素、胞外蛋白酶和细胞毒性肠毒)设计合成4对引物,对2008—2009年广东、江西两省的临床分离菌株进行检测,评价菌株毒力基因的分布特征及其毒力强弱。60株临床分离株中,具有4种毒力基因(aer+hly A+epa+act+)的菌株占58.33%(35/60),是主要毒力基因型;其他25株的毒力基因有不同数量缺失。不同基因型的代表菌株对剑尾鱼攻击试验结果表明,嗜水气单胞菌毒力因子之间表现出协同作用,aer+hly A+epa+act+型的毒力最强,对剑尾鱼的致死率最高。Four main extracellular virulence factors of Aeromonas hydrophila, including aerolysin （ aer ）, hemolysin （ hlyA ） , extracellular protease （ epa ） and cytotoxic enterotoxin （ act ）, were investigated in 60 isolates of A. hydrophila by PCR. These 60 stains were isolated from diseased fishes from Jiangxi and Guangdong province in 2008-2009. The majority （ 58.33% ） of the strains carried the four virulence genes, aer＋hlyA＋epa＋act＋ virulence genotype isolates were major in A. hydrophila isolates . 12 representative strains from these genotypes were randomly selected, and individually injected into swordtail. The results showed that the isolates with more virulence factors had stronger pathogenicity to swordtail, aer＋hlyA^epa＋act＋ genotype isolates were more virulent to the swordtail compared to others.

关 键 词：嗜水气单胞菌 气溶素 溶血素 胞外蛋白酶 细胞毒性肠毒素 毒力基因 剑尾鱼 

分 类 号：S852.61[农业科学—基础兽医学]