光周期对雨生红球藻生物量、虾青素累积和抗氧化能力的影响  

Effects of Photoperiods on Biomass,Astaxanthin Accumulation and Antioxidative Capacity in a Green AlgaHaematococcus pluvialis

作　　者：江红霞[1] 林雄平[2] 雷梦云[3] 孔祥会[1] 

机构地区：[1]河南师范大学水产学院,河南新乡453007 [2]宁德师范学院生物系,福建宁德352100 [3]河南师范大学生命科学学院,河南新乡453007

出　　处：《水产科学》2015年第9期555-559,共5页Fisheries Science

基　　金：河南省重点科技攻关项目(102102210181);河南省水产学重点学科项目(2012)

摘　　要：研究了不同光周期条件(0L∶24D、4L∶20D、8L∶16D、12L∶12D、16L∶8D、20L∶4D、24L∶0D)对雨生红球藻生物量(细胞密度和单位体积干质量)、虾青素含量(单位干质量虾青素含量和单个细胞虾青素含量)、丙二醛含量和总抗氧化能力的影响。生物量的测定采用显微细胞计数法和直接称质量法,虾青素含量、丙二醛含量和总抗氧化能力的测定采用分光光度法。试验结果表明,雨生红球藻生物量和丙二醛含量随光照时间的延长先升后降,虾青素含量和总抗氧化能力则随光照时间的延长逐渐升高;雨生红球藻生物量和丙二醛含量均在16L∶8D光周期条件下达到最大值,且显著高于连续黑暗和连续光照条件下的生物量和丙二醛含量(P<0.05);雨生红球藻虾青素含量和总抗氧化能力在连续光照条件下最高,且均显著高于每日光照时间小于20h条件下的虾青素含量和总抗氧化能力(P<0.05);在20L∶4D和连续光照条件下的雨生红球藻生物量、单个细胞虾青素含量和丙二醛含量无显著差异(P>0.05),但这两种条件下的单位干质量虾青素含量和总抗氧化能力差异显著(P<0.05);在连续黑暗条件下,雨生红球藻生物量、虾青素含量、丙二醛含量和总抗氧化能力均最低。本研究结果为雨生红球藻的大规模培养和虾青素的规模化生产和应用提供了理论基础。The effects of photoperiods （0L ： 24D,4L = 20D,8L ： 16D,12L ： 12D,16L ： 8D,20L ： 4D and 24L ： 0D） on biomass（cell density and dry weight）, astaxanthin content（astaxanthin content perunit dry weight and astaxanthin content per cell）, malondialdehyde content and total antioxidant capacity were investigated in green alga Haernatococcus pluvialis. The biomass was determined by microscopic cells counting method and direct weighing method, and the astaxanthin content, malondialdehyde content and total antioxidant capacity were measured by a spectrophotometric method. The results showed that the biomass and malondialdehyde content were first increased and then decreased, whereas astaxanthin content and total antioxidant capacity increased with the extension of daily illumination time. The biomass and malondialdehyde content reached the maximum values under photoperiod of 16L ： 8D, and were significantly higher than those under continuous darkness and continuous illumination （P〈0. 05）. The maximal astaxanthin content and total antioxidant capacity were obtained under continuous illumination, and were significantly higher than those under illuminations of less than 20 hours per day （P〈0.05）. There was no significant difference in biomass, astaxanthin content per cell and malondialdehyde content between the two groups of algae under photoperiod of 20L = 4D and continuous illumination（P〉0.05）, whereas there was significant difference in astaxanthin content per dry weight and total antioxidant capacity between these two groups of algae （P%0. 05）. The biomass, astaxanthin content, malondialdehyde content and total antioxidant capacity were the minimum under continuous darkness. The findings provide the theoretical basis with the mass culture of H. pluvialis and the large-scale production and application of astaxanthin.

关 键 词：雨生红球藻 光周期 生物量 虾青素含量 抗氧化能力 

分 类 号：S963.213[农业科学—水产养殖]