Notch信号通路相关蛋白在食管鳞状细胞癌中的表达  

Expressions of Notch signaling-associated proteins in esophageal squamous cell carcinoma

作　　者：袁勇[1] 胡杨[1] 赵雍凡[1] 陈龙奇[1] 

机构地区：[1]四川大学华西医院胸外科,成都610041

出　　处：《中华胃肠外科杂志》2015年第9期909-913,共5页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金（30770982）

摘　　要：目的：探讨Notch信号通路相关蛋白在食管鳞癌中的表达及其功能。方法纳入食管鳞癌患者50例，通过免疫组织化学方法检测癌组织及癌旁5 cm以上组织中Notch信号通路4种受体（Notch1、Notch2、Notch3和Notch4）与5种配体（Dll-1、Dll-3、Dll-4、Jagged 1和Jagged 2）的表达情况，并分析上述Notch信号通路蛋白与癌组织细胞增生情况（Ki-67指数）及与患者临床病理特征的相关性。结果食管癌组织中Notch1和Notch2表达水平明显低于癌旁组织（均P＜0．01）；Notch3、Dll-1和Dll-3在癌组织与癌旁组织中表达的差异无统计学意义（均P＞0．05）；而Notch4、Dll-4和Jagged2则在癌组织及癌旁组织中均未见明显表达。 Notch1表达与食管癌淋巴结转移及TNM分期呈显著负相关（均P＜0．01）；Jagged1表达与TNM分期呈显著正相关（P＜0．01）。食管癌组织中Ki-67细胞增生指数明显高于癌旁组织（P＜0．01），其与Notch1和Notch3表达呈显著负相关（均P＜0．01）；而与Dll-1和Jagged 1呈显著正相关（均P＜0．01）。结论 Notch信号通路在食管鳞状细胞癌的发生发展过程中可能起到了抑癌基因的作用，其中Notch1蛋白作用显著。Objective To investigate the expressions and the role of Notch signaling-associated proteins in esophageal squamous cell carcinoma （ESCC）. Methods Fifty patients with ESCC were included in this study. The expressions of Notch signaling-associated protein （4 receptors： Notch1, Notch2, Notch3, Notch4; 5 ligands： Dll1, Dll3, Dll4, Jagged1, Jagged2） in cancer foci and adjacent normal tissues （5 cm distance to cancer） were examined by immunohistochemitry. Correlations of these proteins with cancer cell proliferation （ Ki-67 index ） and clinicopathologic features were investigated . Results Higher levels of Notch1 and Notch2 were measured in cancer foci compared with adjacent tissues （all P〈0.05）. There were no differences in the expressions of Notch3, Dll1 and Dll3 （all P〉0.05）. Notch4, Dll4 and Jagged2 were not detected in both cancer foci and adjacent tissues. Notch1 expression was negatively correlated with lymph node metastasis and TNM staging （all P〈0.01）. Jagged 1 expression was positively correlated with TNM staging （P〈0.01）. Ki-67 index was obviously higher in cancer foci, while it was negatively correlated with Notch1 and Notch3 （all P〈0.01） and positively correlated with Dll1 and Jagged 1 （all P〈0.01）. Conclusion Notch signaling path may act as tumor suppressive gene in the pathogenesis of esophageal squamous cell cancer , in which Notch1 protein plays an important role.

关 键 词：食管肿瘤 鳞状细胞癌 NOTCH信号通路 Ki鄄67指数 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]