G蛋白耦联受体31在结直肠癌组织中的表达及临床意义  

Expression and clinical significance of G protein-coupled receptor 31 in colorectal cancer tissue

作　　者：邹一丰[1] 林绪涛[1] 范德军[1] 陈秀婷[1] 杨峥[2] 郑晓彬[1] 刘炫辉 吴小剑[1] 兰平[1] 

机构地区：[1]中山大学附属第六医院结直肠外科 广东省结直肠盆底疾病研究重点实验室,广州510655 [2]中山大学附属第一医院病理科

出　　处：《中华胃肠外科杂志》2015年第9期935-940,共6页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金（81402412）

摘　　要：目的：探讨G蛋白耦联受体31（GPR31）在结直肠癌患者中的表达及临床意义。方法收集1996年1月至2008年12月间于中山大学附属第一医院接受手术切除并经病理证实的321例结直肠癌患者的癌组织及癌旁正常组织标本。采用免疫组织化学法检测结直肠癌组织和正常黏膜组织标本中GPR31的表达水平并进行比较。根据结直肠癌组织平均吸光度值分界值，将患者分为GPR31低表达组和高表达组，比较两组患者的临床病理特征及预后情况，并分析影响预后的危险因素。结果 GPR31在结直肠癌组织中表达水平明显高于正常黏膜组织[平均吸光度值（0．063±0．014）比（0．045±0．020），P＝0．000]。以0．051的平均吸光度值为分界值将全组患者分为GPR31低表达组197例和高表达组124例。高表达组患者的远处转移率明显高于低表达组[12．1％（15／124）比4．1％（8／197），P＝0．007]。GPR31低表达组和高表达组的5年总生存率分别为84．3％和59．7％，无病生存率分别为82．2％和59．7％，差异均有统计学意义（均P＜0．05）。多因素分析结果显示，高龄、癌胚抗原异常、淋巴结转移、远处转移和GPR31高表达是影响结直肠癌患者总生存率和无病生存率的独立危险因素（均P＜0．05）。结论 GPR31在结直肠癌组织中表达上调，其高表达提示患者预后不良，可作为判断患者预后的参考指标。Objective To investigate the expression and clinical significance of G protein-coupled receptor 31（GPR31） in colorectal cancer tissue. Methods Cancer tissues and adjacent normal tissues of 321 cases with colorectal cancer confirmed by pathology and undergoing resection in the First Affiliated Hospital of Sun Yat-sen University from January 1996 to December 2008 were collected. The expression of GPR31 was examined by immunohistochemical staining. According to the expression level of GPR31 （A value=0.051）, all the patients were divided into low GPR31 expression group and high GPR31 expression group. Clinicopathology and prognosis between the two groups were compared. Risk factors affecting prognosis were investigated. Results GPR31 expression was significantly higher in colorectal cancer tissues compared to adjacent normal tissues （mean A, 0.063±0.014 vs. 0.045±0.020, P〈0.001）. A total of 197 cancer tissue samples were defined as low expression and 124 as high expression. Significant difference was observed in the number of patient in pM classification between the two groups （P=0.007）. High expression group had obviously higher distant metastasis rate than low expression group [12.1%（15/124） vs. 4.1%（8/197）, P=0.007]. The 5-year survival rate and tumor-free survival rate were 84.3% and 82.2% in the low expression group, and both 59.7% in high expression group （all P〈0.05）. Multivariate analysis revealed elderly, abnormal CEA, lymphatic metastasis, distant metastasis and up-regulated GPR31 expression were independent risk factors of overall survival and disease-free survival in colorectal cancer patients （all P〈0.05）. Conclusions GPR31 expression is significantly up-regulated in colorectal cancer tissues. High GPR31expression indicates poor prognosis of colorectal cancer, and may be used as a predictive marker.

关 键 词：结直肠肿瘤 G蛋白耦联受体31 免疫组织化学 预后 

分 类 号：R735.34[医药卫生—肿瘤]