改良CT扫描及CT尿路成像在大鼠膀胱癌动物模型活体检测中的价值  

Significance of modified CT scanning and CT urography for the living body detection of rat models of bladder cancer

作　　者：许天源[1] 朱照伟[1] 夏磊磊[1] 王先进[1] 秦亮[1] 张祥[1] 沈周俊[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院泌尿外科,上海200025

出　　处：《现代泌尿外科杂志》2015年第9期655-658,共4页Journal of Modern Urology

基　　金：国家自然科学基金(No:81472379);上海市科委实验动物研究领域科技支撑项目(No:12140901200)

摘　　要：目的：利用N‐甲基‐N‐亚硝脲（MNU）诱导构建大鼠原位膀胱癌动物模型，并提出一种改良的CT扫描和CT尿路成像（CTU）活体检测方法。方法将30只10～12周龄的SpragueDawley（SD）大鼠随机分为两组，A组10只为对照组，每2周予膀胱灌注枸橼酸缓冲液0．1mL，共4次；B组20只为MNU造模组，予A组同法膀胱灌注MNU溶液0．1mL（2mg）。2组大鼠造模诱导期后均常规饲养，观察一般情况。14周时，利用大鼠膀胱灌注欧乃派克稀释液后CT平扫的改良CT检测方法对存活大鼠进行活体成瘤鉴定，并通过三维重建进行CTU成像。活体检测后处死所有大鼠，取膀胱组织进行病理组织学观察。结果14周时A组大鼠无成瘤；B组成瘤率94％，病理诊断为膀胱尿路上皮癌。所有成瘤大鼠CT扫描均见异常，膀胱肿瘤表现为乳头状、团块状低信号新生物，凸向高信号膀胱腔形成充盈缺损。CTU下，膀胱原位肿瘤表现为椭球表面形状各异的凹陷，大小、边界、位置清晰。结论作者首次提出了一种改良CT及CTU小动物原位膀胱肿瘤检测方法，对膀胱癌动态生长和治疗效果的活体评价和连续监测有巨大价值，值得在今后膀胱癌相关动物实验中应用推广。Objective To evaluate the diagnostic efficacy of a modified CT scanning and CT urography （CTU） in rat or‐thotopic models of bladder cancer induced by N‐methyl‐nitrosourea （MNU） .Methods A total of 30 Sprague Dawley （SD） rats （10-12 weeks old） were randomly divided into the control group （group A ,n=10） and model group （group B ,n=20） . Rats in group A were received intravesical instillation of 0 .1 mL citrate buffer solution every other week for four times .Rats in group B were treated with 0 .1 mL MNU solution （2 mg） every other week for four times .In week 14 ,all rats underwent living body detection using a modified CT method ,which combined CT plain scanning with intravesical instillation of omnipaque solu‐tion .Then CTU was performed using three‐dimensional reconstruction based on CT data .Then all rats were sacrificed for bladder pathological examination .Results In week 14 ,no neoplasms were found in rats of group A ,while bladder tumors classified as urothelial carcinoma were observed in 94% of rats in group B .Using the modified method ,all tumor‐bearing rats showed abnormal CT imaging ,which was characterized by papillary or bulky filling defect in high‐signal background .In CTU imaging ,neoplasms manifested as different forms of concave with clear size ,boundary and location in the ellipsoidal surface . Conclusions In this study ,we established a modified CT scanning and CTU method applying to living body detection of ani‐mal orthotopic bladder cancer for the first time .Our job will contribute to the living body detection and consecutive surveil‐lance for dynamic growth and therapeutic effects of bladder cancer .This CT‐based imaging system is worth to be popularized in animal researches in this field .

关 键 词：膀胱癌 大鼠 MNU CT CT尿路成像 

分 类 号：R737[医药卫生—肿瘤]