Flocculating Properties and Production of the Compound Bioflocculant by Rhizobium Radiobacter F2 and Bacillus Sphaeicus F6  

作　　者：Lixin Li Lingyan Feng Fang Ma Qianshen Zhao 

机构地区：[1]State Key Laboratory of Urban Water Resource and Environment,School of Municipal and Environmental Engineering,Harbin Institute of Technology [2]School of Environment and Chemical Engineering,Heilongjiang University of Science and Technology [3]School of Energy and Civil Engineering,Harbin University of Commerce

出　　处：《Journal of Harbin Institute of Technology(New Series)》2015年第4期20-26,共7页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by the National High Technology Research and Development Program of China(Grant No.2009AA062906);the"Twelfth Five-Year Plan"National Key Technology R&D Program of China(Grant No.2012BAD14B06-04);the National Natural Science Foundation of China(Grant No.51478140&51408200);State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology)(Grant No.2015DX06);Promising Youngsters Training Program of Heilongjiang University of Science and Technology(Grant No.Q20120201)

摘　　要：A compound bioflocculant CBF,produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6, was investigated with regard to its production and flocculating properties. The optimization of the culture medium constituents including carbon source,nitrogen source and C / N ratio,metal ions and ionic strength on CBF production were studied. Flocculating properties of CBF were examined by a series of experiments and CBF had good flocculating activities in kaolin suspension with divalent cations and stable over wide range of p H. Studies of the flocculating properties revealed that the flocculation could be stimulated by cations Ca2+,Mg2+,Fe2+,Al3+and Fe3+. In addition,it was stable at 4-30 ℃ in the presence of Ca Cl2. It was found to be effective for flocculation of a kaolin suspension under neutral and weak alkaline conditions( p H 7. 0- 9. 0),and flocculating activities of higher than 95% were obtained when the CBF concentrations among 6- 14 mg / L at p H 8. 0. The results of this study indicate that CBF is a potential replacement of conventional synthetic flocculants and is widely applied in water treatment and downstream processing of food and fermentation industries.A compound bioflocculant CBF,produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6, was investigated with regard to its production and flocculating properties. The optimization of the culture medium constituents including carbon source,nitrogen source and C / N ratio,metal ions and ionic strength on CBF production were studied. Flocculating properties of CBF were examined by a series of experiments and CBF had good flocculating activities in kaolin suspension with divalent cations and stable over wide range of p H. Studies of the flocculating properties revealed that the flocculation could be stimulated by cations Ca^2＋,Mg^2＋,Fe^2＋,Al^3＋and Fe^3＋. In addition,it was stable at 4-30 ℃ in the presence of Ca Cl2. It was found to be effective for flocculation of a kaolin suspension under neutral and weak alkaline conditions（ p H 7. 0- 9. 0）,and flocculating activities of higher than 95% were obtained when the CBF concentrations among 6- 14 mg / L at pH 8. 0. The results of this study indicate that CBF is a potential replacement of conventional synthetic flocculants and is widely applied in water treatment and downstream processing of food and fermentation industries.

关 键 词：compound bioflocculant Rhizobium radiobacter Bacillus sphaeicus PRODUCTION flocculating activity 

分 类 号：X172[环境科学与工程—环境科学]