白花蛇舌草总黄酮对肝癌MHCC97-H细胞增殖的抑制作用  

Inhibitory effect of total flavones of Oldenlandia diffusa Willd on the proliferation of hepatocellular carcinoma MHCC97-H cells

作　　者：张彦兵[1] 王天昶[1] 朱娇[2] 郭亚焕[1] 肖菊香[2] 廖子君[1] 阮之平[2] 

机构地区：[1]西安交通大学医学院附属陕西省肿瘤医院,陕西西安710061 [2]西安交通大学医学院第一附属医院,陕西西安710061

出　　处：《现代肿瘤医学》2015年第19期2716-2719,共4页Journal of Modern Oncology

基　　金：陕西省社会发展攻关计划(编号:2013K12-07-12)

摘　　要：目的:观察白花蛇舌草总黄酮(FOD)对肝癌MHCC97-H细胞增殖和凋亡的影响。方法:将生长良好的细胞分为空白组、FOD组、5-FU组及联合组四组,分别干预48小时,观察细胞的形态,MTT法检测其对肿瘤细胞增殖的抑制作用;流式细胞仪检测FOD对MHCC97-H肝癌细胞凋亡的影响。结果:FOD对肝癌MHCC97-H细胞有显著的抑制作用,并且具有剂量和时间依赖性,不但能使细胞密度减少,细胞变小,核固缩,而且能显著抑制人肝癌MHCC97-H细胞的增殖并诱导该细胞的凋亡(P<0.05)。结论:白花蛇舌草总黄酮能够通过抑制肿瘤细胞生长、促进肿瘤细胞凋亡,明显抑制肝癌MHCC97-H细胞的增殖。Objective：To observe the effect of total flavonoids of Oldenlandia diffusa（FOD）on the proliferation and apoptosis of hepatocellular carcinoma MHCC97 - H cells. Methods：MHCC97 - H cells were divided into four groups：control group,FOD group,5 - FU group and combined group,intervented for 48 hours,observe the cells form, detect the inhibitory effect on tumor cell proliferation by MTT method and the influence of FOD on apoptosis of hepa-tocellular carcinoma cell line MHCC97 - H by flow cytometry. Results：FOD had significant inhibitory effect on liver cancer MHCC97 - H cells with dose and time dependence,not only can make the cells density decreased,cells be-come small,nuclear pyknosis,and inhibit the apoptosis of hepatocellular carcinoma MHCC97 - H cells proliferation and induce cell apoptosis（P〈 0. 05）. Conclusion：Total flavones of Oldenlandia diffusa willa can inhibit the prolifer-ation of hepatocellular carcinoma MHCC97 - H cells by inhibiting the growth of tumor cells and promoting the apopto-sis of tumor cells.

关 键 词：白花蛇舌草总黄酮 MHCC97 - H 细胞 细胞凋亡 

分 类 号：R73[医药卫生—肿瘤]