枸杞多糖对大鼠脑胶质瘤细胞株 C6增殖及凋亡的影响  

Experimental Study of Proliferation Inhibition and Apoptosis Mechanism of Lycium Barbarum Polysaccharides on C6 Glioma Cells in Rats

作　　者：董静[1] 单铁英[2] 潘秀兰[2] 韩艳珍[3] 赵沙河 王鸣飞 刘凤丽[2] 袁征[2] 

机构地区：[1]河北省邯郸市疾病预防控制中心,河北邯郸056002 [2]河北工程大学,河北邯郸056002 [3]河北工程大学附属医院,河北邯郸056002 [4]河北省永年县第一医院,河北永年056002 [5]河北省邯郸市妇幼医院,河北邯郸056002

出　　处：《医学临床研究》2015年第8期1610-1612,共3页Journal of Clinical Research

摘　　要：【目的】探讨枸杞多糖（lycium barbarum polysaccharides,LBP）对大鼠脑胶质瘤细胞株 C6增殖及凋亡的影响。【方法】在倒置显微镜下观察脑胶质瘤细胞凋亡的形态学变化；MTT 法检测 LBP 对 C6细胞增殖抑制率的变化；用流式细胞仪检测细胞凋亡率和细胞周期的变化；免疫细胞化学法测定 C6细胞中增殖细胞核抗原（proliferating cell nuclear antigen,PCNA）的表达变化。【结果】经 LBP 诱导后的 C6出现细胞形态不规则,变圆并脱落的现象；LBP 能抑制 C6细胞的增殖能力,且呈剂量依赖性；LBP 作用后 C6的细胞周期更多地被阻止于 G2/M 期；LBP 作用后的脑胶质瘤 C6细胞中 PCNA 表达水平下降。【结论】LBP 可抑制大鼠脑胶质瘤 C6细胞增殖并使其凋亡,其作用机制可能是 LBP 可下调大鼠脑胶质瘤 C6细胞 PCNA 的表达水平。[Objective]To explore the proliferation inhibition and apoptosis mechanism of Lycium barba-rum polysaccharides （LBP）on C6 glioma cells in rats.[Methods]The morphological changes of apoptosis was observed by inverted microscope.And the proliferation inhibitory rate was detected by methyl thiazolyl tetrazo-lium （MTT）.Cell apoptosis rate and cell cycle were detected by flow cytometry.The expression level of prolif-erating cell nuclear antigen （PCNA）was detected by immunocytochemistry.[Results]The cells became irreg-ular-shaped,round and sloughed off by the induction of LBP.And LBP could inhibit the cell proliferation abili-ty in a dose dependent fashion.Cell cycle was arrested in G2/M phase.And there was a down-regulation of PC-NA.[Conclusion]LBP can inhibit the cell proliferation and apoptosis.And the mechanism may be through a LBP-mediated down-regulation of PCNA in rat C6 cells.

关 键 词：多糖类/药理学 枸杞子/药理学 脑肿瘤/病理学 神经胶质瘤/病理学 肿瘤细胞 培养的 细胞增殖 细胞凋亡 

分 类 号：R730.264[医药卫生—肿瘤]