解淀粉芽孢杆菌抑菌活性初步研究  

Study on Antifungal Substance Produced by Bacillus amyloliquefaciens

作　　者：杨帆[1] 李新民[1] 刘春来[1] 王爽[1] 夏吉星[1] 王克勤[1] 刘兴龙[1] 邵天玉[1] 徐充[1] 

机构地区：[1]黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2015年第9期55-59,共5页Heilongjiang Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)资助项目(2011AA10A205);黑龙江省农业科技创新工程资助项目(2014QN009)

摘　　要：为了进一步分离纯化抑菌蛋白和脂肽类物质，促进拮抗菌的开发和利用，选取2株对多种植物病原真菌具有良好拮抗作用的解淀粉芽孢杆菌，利用硫酸铵沉淀法粗提2株菌株的抑菌蛋白，酸沉淀法粗提脂态类抑菌物质，分别测定粗提物对多种植物病原真菌的抑制效果。初步检测2株菌株是否具有产纤维素酶、蛋白酶和嗜铁素活性。结果表明：BQD1菌株20％～30％的蛋白粗提液对玉米大斑病菌拮抗作用最强，抑菌直径达31．48 mm。 TY 菌株30％～40％的蛋白粗提液对玉米大斑病菌抑菌直径最高达34．20 mm。2株菌株脂态类粗提液对靶标病原菌株的拮抗作用均高于其发酵液，且均可产生纤维素酶、蛋白酶和嗜铁素。In order to isolate and purify the antifungal proteins and lipopeptides products ,and promote the devel‐opment and utilization of antagonistic bacteria ,two strains of Bacillus amylolique f aciens which showed obvi‐ous antifungal activity against plant pathogens were selected .Antifungal proteins of two strains were prelimina‐ry separated ,using the methods of ammonium sulfate precipitation ,and tested on plant pathogens .Antifungal activity of lipopeptides products which extracted with methanol from hydrochloric acid precipitate were tested plant pathogens .Two strains were also tested wether could produce cellulose ,protease and siderophore .The re‐sults showed that the strongest antifungal proteins inhibition activity of strain BQD1 was under the ammonium sulfate condition of 20% ~ 30% ,its diameter of inhibition zone to Setosphaeria turcica was 31 .48 mm .Anti‐fungal proteins inhibition activity of strain TY was under the ammonium sulfate condition of 30% ~ 40% ,its diameter of inhibition zone to Setosphaeria turcica was 34 .20 mm .Lipopeptides products of two strains had stronger antagonism effect on target plant pathogens than its ferfentation .two strains could both produce cellu‐lose ,protease and siderophore .

关 键 词：解淀粉芽孢杆菌 抑菌活性物 植物病原真菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治]