超临界CO_2流体萃取滇红茶及其提取物成分分析  

Supercritical CO_2 Extraction of Dianhong Tea and Analysis of Its Extract Components

作　　者：孙海林[1] 阮志国[1] 车彦云[1] 黄卫东 

机构地区：[1]云南中医学院,云南昆明650500 [2]云南白药天颐茶品有限公司,云南昆明650034

出　　处：《云南中医学院学报》2015年第4期23-27,37,共6页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：云南白药;云南中医学院中医药教育创新基金项目(YB2012J03)

摘　　要：目的考察超临界二氧化碳提取滇红茶香气成分的最佳工艺,并对提取物成分进行分析。方法采用单因素试验考察夹带剂、茶叶粒度、萃取时间、萃取压力、萃取温度对提取率的影响,再通过正交试验优化工艺条件;用GC-MS方法进行化学成分分析。结果最佳工艺条件为:夹带剂250m L无水乙醇、茶叶粒度20目、提取时间0.5h、萃取压力25MPa、萃取温度45℃,萃取率为0.46%,提取物经GC/MS检测到近50种成分。结论超临界CO2流体萃取技术可用于滇红茶有效成分的提取。Objective To optimize the supercritical CO2 extraction procedure of Dianhong Tea, and analyze the components of extracts. Methods The effect factors including entrained, the feel particle, extraction time, extraction pressure and the extraction temperature were investigated by single factor method, then through the orthogonal tests optimize processing conditions, the extract were identified by GC-MS. Results The optimum extraction parameters were established with the entrainer of 250 mL ethanol, the feel particle of 20 meshes, the extraction time of 0.5 h, the extraction pressure of 25 MPa, the extraction temperature of 45℃, the yield was up to 0.46%, and 50 kinds of components were identified. Conclusion Supercritical fluids extraction can be used to extract the effective components of Dianhong Tea.

关 键 词：超临界CO2 滇红茶 GC-MS 

分 类 号：R284.2[医药卫生—中药学]